inhibited gene 中文意思是什麼
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Treatment of mature green fruits with 0. 1 mm aba enhanced the ethylene production and gene expressions of leerfl, leerf2. treatment of mature green fruits with 1 mm sa had slight inhibition on ethylene production. sa treatment inhibited the expression of leerf1 and had no effect on the expression of leerf2
激素和化學試劑處理結果表明： 0 . 1mmaba處理綠熟期普通番茄果實8h后，乙烯生成增加， leerf1 、 leerf2基因表達增強； 1mmsa處理綠熟期普通番茄果實8h后，乙烯的生成稍有抑制， leerf1基因表達受到抑制， leerf2基因表達與對照基本相近；而aba和sa處理對pti4基因的表達沒有影響，表明果實中pti4基因表達不受aba和sa的誘導。 -
Induction of pal activity, saponin synthesis and sqe gene expression were all inhibited by such inhibitor treatments and reduced by incubation with catalase and h2o2 scavengers
激發子誘導胞內鈣離子的升高以及過氧化氫的產生，對于激發子誘導的抗性相關基因的轉錄是必須的。 -
Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999, and its molecular functions were not well understood. it mainly expresses in terminally differentiated cells. by transfection of restin into tumor cell, the cells were arrested in g1 phase and the cell proliferation was inhibited
Restin是1999年本室在研究全反式維甲酸誘導細胞分化時克隆得到的一個人類未知功能的新基因，該基因主要表達于終末分化細胞，穩定轉染該基因的細胞出現g1期阻滯，抑制細胞的增殖，提示restin與細胞周期的調控有關。 -
Our data demonstrated that extracellular ca2 + was required for blue - light - induced anthocyanin accumulation and chs gene expression and verapamil sensitive plasma membrane ca2 + channel played a positive effector on bl response. heparin, an antagonist of ip3 receptor increased the anthocyanin content induced by bl but inhibited chs gene expression
Ip3與cs通道受體結合抑制劑肝素處理可促進藍光誘導的花色素苦積累，但抑制chs基因表達； ip3分解抑制劑lic13則抑制花色素著積累而促進基因表達；施加肌醇既促進藍光誘導花色素著積累又促進基因的表達。 -
The results indicate that : in both normal temperature and heat shock, the contents of mitochondrion proteins of sterile plants and control plants are significantly different ( p < 0. 01, n = 10 ) ; the differences of contents of mitochondrion proteins of sterile plants in the normal temperature and heat shock response are not significant, which mean that the expression of hsp70 gene is inhibited and the function of mitochondrion is disturbed
01 ， n 10 ） ；而不育植株在常溫和熱激條件下線粒體蛋白含量差異不顯著，說明轉化株中hsp70基因的表達受到抑制，從而進一步擾亂了線粒體的功能，最終導致轉化株雄性不育，表明hsp70反義cdna能夠通過抑制hsp70基因的表達導致植物雄性不育。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。 -
Chs gene expression induced by bl was enhanced by a23187 and inhibited by egta and verapamil. but the effect of another ca2 + channel blocker nif on the gene expression was opposite to its role in the inhibition of anthocyanin accumulation
A23187對藍光誘導的chs基因表達有一定的促進作用， egta和異博定則有抑制作用；尼群地平（ nif ）對花色素著積累和基因表達的作用不一致，需要進一步研究。 -
Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1
Hsp70是一種分子伴侶，主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復，對線粒體結構和功能發揮重要作用，已有研究證明hsp70與動植物的發育有密切的關系，本研究將hsp70正、反義cdna構建成表達載體，並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草，試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株，以證明hsp70反義cdna能創造雄性不育系。
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