isolated cell 中文意思是什麼

isolated cell 解釋
分離細胞
  • isolated : 被隔離的
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system

    曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。
  2. Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively

    有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域，目前該領域的研究大多集中在利用分離的酶進行生物催化，利用微生物完整細胞進行的研究比較少。
  3. Other spiroplasmas have been isolated and cultured on cell-free media.

    其它螺原體已被分離出來，並在無細胞的培養上培養。
  4. Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells. in vitro, es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers

    胚胎幹細胞（ embryonicstemcell ， escell ）系是從早期胚胎細胞分離培養而建立的高度未分化的具有全能性的細胞系。
  5. The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage

    對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究，應用溶媒萃取、硅膠柱分離等方法，從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ，以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。
  6. An isolated cell comes close to satisfying the likeness of a zygote.

    一個分離的細胞接近達到合子的外貌。
  7. Glial cell line - derived neurotrophic factor ( gdnf ) was originally isolated based on its potent and specific ability to promote the survival and morphological differentiation of dopaminergic neurons and motoneurons in embryonic midbrain cultures. in addition, gdnf also support the survival and regulate the differentiation of many peripheral neurons, including sympathetic, parasympathetic, sensory and enteric neurons. gdnf also plays a crucial role outside the nervous system, as a morphogenetic factor in kidney development and as a regulator of spermatogonia differentiation

    Gfr 1與gdnf結合位點的研究膠質細胞源性神經營養因子（ gdnf ）對多巴胺能神經元、運動神經元、感覺神經元、腸道神經元等多種神經元具有促進存活及保護作用，它還能促進腎臟的發育和精原細胞的成熟，因此，極有希望用於治療神經損傷和神經系統退行性病變。
  8. The isolated hepatocytes, isolated perfused organs, tissue slices, liver cell lines, subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays, especially the isolated hepatocytes

    目前，常用的肝臟體外模型包括原代肝細模型、離體肝臟模型、肝臟切片模型、肝細胞系模型、亞細模型及基因工程細模型等，其中原代肝細型最?常用。
  9. Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance. this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell. the sod2 gene was isolated from s. pombe by rt - pcr method and confirmed by sequencing

    雖然sod2基因已經克隆，但是sod2在高等植物中異源表達及對其影響還未見報道，因此sod2基因在擬南芥中過量表達，對研究細胞的離子均衡及運用基因工程手段有效地提高植物的抗逆性具有重要意義。
  10. There is considerable evidence that sycamore cell-wall xyloglucan is the same as that isolated from seps.

    相當多的證據證明槭細胞壁木葡聚糖與從SEPS分離出來的是相同的。
  11. Plants are thought to remove na + from the cytoplasm by transporting it into the vacuolar or out of the cell using na + / h + exchangers localized in the vacuolar and plasma membranes, respectively. sos1 encoding a plasma membrane na + / h + antiporter and atnhxl encoding a vacuolar na + / h + antiporter were isolated from glycophytic arabidopsis thaliana, and overexpression of atnhxl and sos1 in arabidopsis thaliana increased the salt tolerance of transgenic plants significantly

    目前，擬南芥細胞內控制na ~ +外排的基因sos1及離子區隔化基因atnhx1均已克隆， sos1及atnhx1在擬南芥中的過量表達顯著提高了轉基因植株的耐鹽性，開創了降低na ~ +毒害的基因操作新途徑。
  12. The purposes of the present study were to investigate ( 1 ) the hemodynamic effects of agmatine in anaesthetized dahl salt - sensitive ( ds ) hypertensive and dahl salt - resistant ( dr ) rats ; ( 2 ) the effect of agmatine on vascular tension in the isolated aortic artery of rats and the underlying receptor mechanism ; ( 3 ) the effects of local injection of agmatine on femoral, renal, and mesenteric vascular beds by constant flow perfusion method ; ( 4 ) the effect of agmatine on l - type calcium current ( / ca - t ) in rat ventricular myocytes with whole - cell configuration of the patch - clamp technique ; ( 5 ) the effects of agmatine on free intracellular calcium concentration ( ca2 + d of isolated rat ventricular myocytes

    （ 3 ）採用後肢、腎臟和腸系膜動脈在體恆流灌注法，觀察向灌流環路中直接注射胍丁胺的血管效應。 （ 4 ）應用全細胞膜片箝技術，觀察胍丁胺對大鼠心室肌細胞l -型鈣通道電流（ i _ （ ca - l ） ）的影響。 （ 5 ）用fluo3 - am負載分離的大鼠心室肌細胞后，由激光共聚焦法測定單個心室肌細胞[ ca ~ （ 2 + ） ] _ i的熒光強度，觀察胍丁胺對分離大鼠心室肌細胞內游離鈣濃度（ [ ca ~ （ 2 + ） ] _ i ）的影響。
  13. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  14. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原，對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定，證明該分離病毒與細小病毒科（ parvovirdae ）細小病毒屬（ parvovirus ）的雞細小病毒（ chickenparvovirus ）特性基本相符，核酸型為dna型。
  15. Exosomes derived from human intestinal epithelial cell ( iec ) could be released from apical and basolateral sides and expressed mhc class i molecules, cd26, cd63 in basal conditions besides mhc class ii in inflammatory conditions. the data showed exosomes might be a mechanism of oral tolerance. exosome - like vesicles isolated from rat iec line, which were pulsed by antigens, were shown to induce antigen - spec

    模型的建立和exosomes的分離與純化我們利用一種c57bl / 6小鼠的3lllewis肺癌細胞系作模型，將3ll細胞皮下注射到c57bl / 6小鼠，待3周時無菌取出腫瘤組織，剪碎後用dmem加10 %的血清在37 ， 5 %的czo中培養， 30小時后收集培養上清。
  16. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  17. Secondly, to observd the immunity effects of the dcs ( pcea ) inoculating against to tumors ( hcea + ). methods 1 in intro preparation human mode and tumor cell the peripheral blood mononclear cells ( pbmc ) were isolated from human fresh peripheral blood

    人外周血dc的培養及腫瘤細胞的培養：取健康成人新魚外周全血獲得人外周血單個核細胞（ peripheralbloodmononuclearcell ， pbmc ） 。
  18. A lectin named pclii with multi - functions was isolated from the rhizomes of polygonatum cyrtonema hua, a liliaceae plant used as traditional chinese herbal medicine. pclii can bind mannose and silia acid, it also can agglutinate rabbit erythrocytes and gastric tumor cell lines sgc and hsc strongly and inhibite the growth of some pathogens. pclii is a strong mitogen toward human t - lymphocytes and an efficient ca2 * antagonist ( ca ) or ca2 + channel blocker ( ccb )

    百合科植物囊絲黃精（ polygonatumcyrtonemahua . ）是我國傳統中草藥，其根狀莖中含有一種凝集素，命名為黃精凝集素（ polygonatumcyrtonemahua . lectin ，簡稱pcl ） ，具有特異性結合甘露糖和唾液酸以及凝集兔紅細胞、促t細胞有絲分裂等活性。
  19. Effects of radix morindae officinalls on ex vivo amplification of cd34 cell isolated from cord blood

    中藥巴戟天對晶狀體醛糖還原酶的抑制作用
  20. Moreover, although the diversity degree is limited, the interference level is closed to ideal case ( ie., signal can received by all base stations ) and then capacity closed to isolated cell because of propagation attenuation especially in lower shadowing conditions

    此外，由於信號固有的傳播衰落，雖然分集度受限，但其基站所受干擾水平接近理想情形（移動臺信號被所有基站接收） ，進而容量接近孤立蜂窩的容量。
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