kanamycin 中文意思是什麼

kanamycin 解釋
n. 名詞 【藥學】卡那黴素。

  1. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ,為了解決轉基因裙帶菜的篩選問題,進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗,包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素,除草劑類的basta ,氨甲喋呤類的氨甲喋呤,結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感,對basta非常敏感。
  2. Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad

    對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測,其中2株呈陽性,證明vp4基因已整合到苎麻基因組dna中。
  3. Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. leaves of adventitious shoots were detected by gus staining

    Sin旮16ea )上暗培養3天後,移入加有抗生素150mg ikan和500mg幾cr的誘芽培養基上誘導不定芽的分化。
  4. Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root

    分化出的不定芽切下半張葉片進行gus染色,呈陽性的植株從外植體上切下,在生根培養基( ms 0刀sing lnaa 50mg幾kan )中誘導根的形成。
  5. The results indicated that all isolates exhibited a susceptibility to amikacin and ceftriaxon, and 67 isolates showed a greater or lesser degree resistance to streptomycin, kanamycin, gentamicin, ampicillin, tetracycline, chloramphenicol, florfenical, cefotaxime, cephalothin and ceftiofur, to which 22 isolates exhibited a susceptibility. some isolates showed resistance to multiple antibiotics and displayed a highest level resistance to streptomycin with a frequency of 43. 8 %, followed by tetracycline with a frequency of 30. 3 %

    結果表明,所有分離菌株均對阿米卡星和頭孢曲松敏感; 67株對鏈黴素、卡那黴素、慶大黴素、氨芐西林、四環素、氯黴素、氟苯尼考、頭孢噻肟、頭孢噻吩、頭孢噻呋表現出不同程度的耐藥性,其中對鏈黴素的耐藥率最高,為43 . 8 ,其次為四環素( 30 . 3 ) ,其餘22株為敏感菌株。
  6. Has he ever received streptomycin or kanamycin injections

    他注射過鏈黴素或卡那黴素嗎?
  7. Co. kanamycin nasal drops

    復方卡那黴素滴鼻劑
  8. Method for the determination of kanamycin residues in meat for export. cylinder plate method

    出口肉品中卡那黴素殘留量檢驗方法.杯碟法
  9. Three methods for rapid selection of transgenic cotton plants in laboratory with kanamycin as indirect marker

    標記基因的轉基因抗蟲棉室內快速鑒定方法
  10. Ultrastructural change of chicken basilar papilla during a prolonged administration of kanamycin

    延長卡那黴素外環境持續存在時間雞基底乳頭的超微形態變化
  11. The coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into vector pbi121 containing intron - kanamycin

    再生苗經過戍r和pcrs 。 : : hernbl 。 t分子檢測證明獲得了煙草轉基因植株。
  12. A number of kanamycin resistant transformants were obtained two months later. they were morphologically normal, but grew slower comparing with the control tobacco

    2個月後獲得了一批卡那黴素抗性煙草植株,抗性植株大田生長表型正常,生長速度相對緩慢。
  13. The suitable concentration of the selective agent kanamycin is 50mg. l - 1. the duration of infection and co - culture with agrobacterium influences soybean transformation evidently

    農桿菌侵染時間和共培養時間對大豆的轉化有明顯的影響,侵染時間以15min為宜,共培養時間以3d為宜。
  14. As a selectable agent, concentration of kanamycin is different with medium of buds induction. concentration of kanamycin is higher in the medium that has higher frequency of buds induction

    在不同的出芽培養基上,卡那黴素的選擇濃度也不同,出芽率越高,卡那黴素所需的濃度越高。
  15. From total 16, 660 dipped inflorescences, l, 268g matured seeds were harvested. in kanamycin - resistent experiment the surface - sterilized non - treated seeds were placed on ms - medium with various kanamycin concentrations

    從總共16 , 660個進行了轉基因操作的花序上共收獲1 , 268克成熟種子。
  16. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株( agrobacteriumtumefaciens ) ,含質粒pyhy (目的基因及npt 、 gus基因) ,對白樺試管苗莖段,葉柄,葉片三種外植體進行侵染,結果表明:大葉片生長勢強,為轉基因的最優外植體,轉化率能夠達到22 。
  17. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因( ssnhx1 )的表達載體prok2導入大豆子葉節中,經過含km的篩選培養基連續篩選,獲得了ssnhx1轉基因植株,篩選劑卡那黴素的適宜濃度是50mg . l ~ ( - 1 ) 。
  18. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌后,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。
  19. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中,導入根瘤農桿菌gv3101后,由花浸泡法進行擬南芥遺傳轉化,轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素( 25mg )的ms培養基上篩選,獲得t ;代轉基因植株。
  20. The main factors influencing agrobacterium - mediated genetic transformation of portulaca grandiflora hook, are as follows. the concentration of kanamycin of devision leaves and the calli were 100mg / l and 200mg / l, respectively. calli infection can gain more gus gene transient expression and resistant tissuses than devision leaves

    對松葉牡丹進行遺傳轉化研究發現,採用葉片作為外植體時, kan的濃度100mg / l就可抑制愈傷的誘導,而用愈傷組織作為材料感染時濃度要達到200mg / l才可。
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