leader peptide 中文意思是什麼
leader peptide
解釋
前導肽-
In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon
序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。 -
Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。 -
A gus report gene, gained by the method of pcr, was inserted into the pet22b vector between the sites of hindiii and xho i to construct a transitional vector named pet22g then the signal peptide ( pelb leader ) in the pet22g vector was replaced by a containing grb - ast signal peptide fragment to construct expression vector pet22asg which had been transformed into expression host e. coli bl21 ( de3 ) plyss
先將pcr獲得的gus報告基因插入到經hind和xho雙酶切的pet22b中，構建了中間載體pet22g ；然後再將pcr克隆的一含ast信號肽的片段as取代pet22g上的信號肽pelbleader序列，構建了大腸桿菌分泌表達載體pet22asg 。 -
Effects of different leader peptides on the foreign peptide displayed on gene 8 protein of filamentous phage
不同的引導肽對絲狀噬菌體基因8蛋白展示外源多肽的影響
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