lentogenic strain 中文意思是什麼
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Newcastle disease is a highly contagious and economically most important disease of poultry. the disease also inflicts many other avian species. the disease varies in degree of severity ranging from mild, inapparent infecion to severe disease. based on the severity of the disease in chickens, the causative agent of the disease, newcastle disease virus ( ndv ), is divided into three pathotypes : ( 1 ) the lentogenic strains, which cause mild inapparent infection ; ( 2 ) the mesogenic strains, which cause mild respiratory symptoms with low mortality ; ( 3 ) the velogenic strain, which are highly virulent and cause high mortality
新城疫（ newcastledisease ， nd ）是由新城疫病毒（ newcastlediseasevirus ， ndv ）引起的侵害禽類的急性高度接觸性傳染病，是家禽傳染性疾病中危害最嚴害的疫病之一，由於對世界養禽業危害極大，故被國際獸疫局確定為類傳染病。在傳染性疾病的預防中，疫苗是減少易感人群而被最廣泛採用和最有效的途徑。 -
All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function
本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。 -
It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。
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