leukocyte culture 中文意思是什麼
leukocyte culture
解釋
血沉棕黃層細胞培養-
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上,構建了4個全長基因的分子克隆,分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ,其中兩個轉移到低拷貝載體plg338上,命名為plgd30343 、 plgd70344 。 -
The eiav - pok8. 2 - his was transfected into the donkey leukocyte culture. following an incubation period, reverse transcriptase activity was detected in cell culture supernatants. cytopathic effect was observed by no. six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8. 2 - his
提取前病毒dna , pcr擴增p1p4片段,亞克隆至pmd18 - t載體,測序證明前病毒dna含有六個組氨酸,從而在體外獲得了感染性克隆病毒株。
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