ligation method 中文意思是什麼

ligation method 解釋
結扎法
  • ligation : n. 1. 綁扎。2. 【醫學】結扎；縛法；結扎線，縛線。
  • method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
  1. Method : experimental rat gastric ulcer models including waterlogging stress - restraint ulcer, pyloric ligation induced ulcer, indomethacin induced gastric mucosa injury and absolute alcohol induced gastric mucosa ulcer were used in this experiment

    方法：用水浸應激型、幽門結扎型、消炎痛致胃粘膜損傷型及無水乙醇致胃粘膜損傷潰瘍模型方法試驗。
  2. Artificially using t - a cloning techniques and initiated one - step ligation of multi - fragments method were the main factors in the process of full - length cdna construction

    T - a克隆的巧妙運用和多片段一步連接法的成功運用是全長cdna得以快速構建的主要原因。
  3. Aim to evaluate the feasibility of one simple method to establish model of venous thrombosis for studying the therapeutic effect of intracavitary ultrasonic therapy on venous thrombus of animal models. methods the lower limbs of 20 dogs were divided randomly into the experimental group and the control group. the femoral veins of the experimental group were ligated at the close and distant end respectively to slower the flow of blood. the veins in control group were operated but not ligated. then, the changes of the dogs ' lower limbs were observed and the femoral veins were excised for pathological examinations and examined to investigate the condition of thrombis in the veins at the 1st, 4th and 7th day respectively after operation. results all the dog ' s lower limbs in the experimental group swelled and were lame slightly, the thrombus came forth in all the 6 veins by pathologic study at the 1st day after operation. and it was opposite in the control group. in addition, the swelling of all the dogs ' lower limbs was aggravated and all the 14 femoral veins were filled with compact mixed thrombus at the 4th and 7th day after operation. and it was also opposite in the control group. conclusion the method to establish models of venous thrombosis by the simple ligation of close and distant end of the femoral veins can make thrombosis more approaching clinical course of thrombosis and is satisfying

    目的為研究腔內超聲溶栓對動物模型靜脈血栓的療效而評價一種制備靜脈血栓模型方法的可行性.方法犬20隻採用自身對照研究，犬一側後肢股靜脈為實驗側，另一側為對照側.實驗側行股靜脈近、遠端分別結扎，人為造成犬後肢股靜脈血流緩慢;對照側行手術，但不結扎血管.然後于術后第1 ， 4 ， 7天分別觀察犬後肢變化，切取血管標本做病理觀察，了解血栓形成情況.結果術后第1天實驗側全部出現後肢腫脹，輕微跛行，病理切片顯示: 6條靜脈全部都形成血栓;對照側沒有出現後肢腫脹及跛行， 6條靜脈都無血栓形成.第4 ， 7天實驗側後肢腫脹加重，跛行，病理切片顯示:實驗側14條靜脈血栓充滿管腔，為混和血栓;對照側沒有出現後肢腫脹及跛行， 14條靜脈無血栓形成.結論採用靜脈單純結扎法制備犬靜脈血栓模型，血栓形成更接近臨床血栓形成過程
  4. 2. pcr products can be purified by ctab method, which removing the dntp and primers. the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency

    2 、採用ctab進行pcr產物的純化，以去除pcr產物中的引物和dntp ，所得產物可用於與t載體連接反應，以提高連接效率。
  5. Long - term follow - up of quot; sandwich quot; method of combinded endoscopic band ligation and injection sclerosis in treatment of esophageal variceal bleeding

    套扎及硬化術治療食管下段胃底靜脈曲張15例分析
  6. Long - term follow - up of quot; sandwich quot; method of combined endoscopic band ligation and injection sclerosis in the treatment of esophageal variceal bleeding

    套扎與硬化夾心聯合法治療食管靜脈曲張出血的長程隨訪
  7. The shotgun method was only used with b. subtilis hn503. the optimun conditions for total dna extraction, enzyme digest, ligation and transformation were studied the results showed that the most important factor was transformation efficiency. since the concentration of ligation products was low in common

    在已知基因序列的條件下用pcr和rt一cr克隆基因時，對pcr和rt一pcr的反應條件、產物的回收、回收產物的酶切、酶連和轉化等條件進行了一系列的比較研究。
  8. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
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