linking protein 中文意思是什麼
-
The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
Talin is a important f - actin binding protein, two functions of intact talin are the control of actin filament length and the cross - linking of actin polymers. the binding of f - actin to talin significantly increases actin filament stiffness
Talin是一個重要的f - actin結合蛋白，它可以控制微絲的長度，並且同actin聚合體發生交聯， f - actin和talin發生的結合可以顯著地增加微絲骨架的強度。 -
To those chrome - containing leather wastes, it was difficult to extract high quality collagen protein from them because of the cross linking of chrome between collagen chains, which result in the wasting of useful collagen protein and chromium resources
而含鉻廢棄物中由於膠原與鉻以共價鍵牢固結合，很難將其分離和提純，目前尚未實現其高值利用。 -
A novel protein immobilization approach based on mercapto - pha sam for piezoelectric immunosensors has been proposed, which is formed by coupling mercaptoprorionic acid ( mpa ) to poly ( allylamine hydrochloride ) ( pha ) via 1 - ethyl - 3 - ( 3 - dimethylamino - propl ) carbodiimide ( edc ) and n - hydroxysuccinimide ( nhs ). after self - assembling a mercapto - pha sam on the gold electrode of the quartz crystal, the goat - anti - igm antibody is immobilized via cross - linking with glutaraldehyde. the detecting linear range of the piezoelectric immunosensor employing mercapto - pha sam is 0. 66 - 26. 4 ug / ml
應用偶聯試劑碳二亞胺（ edc ）和n -羥基琥珀酰亞胺（ nhs ）使聚丙烯胺（ pha ）與巰基丙酸（ mpa ）偶合形成巰基化的聚丙烯胺，應用這一新方法，實現了羊抗人igm抗體分子的固定，並在0 . 66 26 . 4 g / ml范圍內對人血清免疫球蛋白igm進行了測定。 -
These reaction products exhibited fluorescent spectra ( 400nm - 490nm ) akin to those of extracts from liposcin - rich tissues. considering that dehydroascorbic acid and mda are active in biological system, the oxidation - enhanced carbonyl - protein cross - linking is suggested to be an important chemical reaction which may take place during ageing and be involved in lipofuscinogenesis
氧化asa的化學性質非常活潑又是常見的還原酮，在我們的實驗中氧化的維c與谷氨酰氨也生成類似的熒光，所以asa與gln生成交聯產物的反應可能與衰老過程中脂褐素的生成相關。 -
In the previous work, we cloned sh2a gene in this region by exon trapping and exon linking, which is a novel member of sh2 family. sh2 domain is considered as " protein recognized code "
在前期工作中，運用外顯子捕獲和外顯子拼接等策略，我們在這一區域克隆了sh _ 2a基因，是sh _ 2信號蛋白家族的新成員。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
分享友人