long reading frame 中文意思是什麼
long reading frame
解釋
長閱讀框架-
The sequence 56 analysis shows that there is a long open reading frame in it which encode a protein of 364 animo acid residues. the m protein include 47 basic amino acid, 30 acidic amino acid, which cause a basic estimated pi of 9. 7. phylogenetic tree based on ndv matrix protein gene shows that the b95 and v4 has the most close relationship than other ndv strain
關于ndvm蛋白國內未見有報道，國外對此蛋白的研究也十分有限，本文克隆出了ndv株b95m蛋白基因的全序列，為m蛋白的表達及進一步研究其在病毒復制與致病中的作用機理打下了基礎。 -
In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail
3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。 -
The cdna is 2 149 bp long with an open reading frame of 1 697 bp, which encodes a polypeptide of 565 amino acids, preprotein of 62. 3 kda ; 5 " untranslated regions contains 59nt, 3 " untranslated regions contains 391nt, a poly ( a ) tail signal and long poly ( a ) tail
該cdna全長2149bp ，包含一個1697bp的開放閱讀框架，編碼565個氨基酸。在起始密碼子上游有一個由59個堿基組成的5 』非編碼區；在終止密碼子下游有一個由391個堿基組成的3 』非編碼區，包括4個分解信號、 1個加尾信號和1個長度為17個腺苷酸的poly （ a ）尾。 -
The gene fragment we have got is 1311bp long, which contains a length of 543bp from 5 ' - end to the coding region, 588bp of open reading frame including " the coding region of 585bp and a termination codon as well as the 3 ' - end sequence of 180bp rich in at ( accounting for 70 % )
從已有的5 』部分序列到該過敏蛋白編碼區為543bp ，編碼181個氨基酸。開放閱讀框588bp ，包括編碼區的585bp及一個終止密碼，可編碼一個由195個氨基酸組成的功能蛋白。 3 』端非編碼區包括180個堿基，富含at （ 70 ） 。
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