low copy number 中文意思是什麼
low copy number
解釋
拷貝數-
Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5
Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移,但似乎不能夠在鏈黴菌fr - 008中復制,因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。 -
Dnd phenotype could be detected when the entire dnd cluster was integrated in the chromosome or carried by the low copy - number plasmid in zx1. however, when dnd cluster was carried by a plasmid with a copy - number around or higher than c. 10 copies in zx1, dnd phenotype could not be observed
當完整的dnd基因簇整合在zx1染色體上或由1 2個拷貝的自主復制的質粒攜帶進入zx1 ,都能在zx1中檢測到dnd表型,但是由10個或超過10個拷貝的質粒攜帶進入zx1 ,就檢測不到dnd表型。 -
Hdgs induction by progeny containing a low copy number of the vector was also dramatically higher than induction by single copy vectors. the reduction of gfp protein and mrna abundance, and the presence of 21 - 26 nt sirnas could be simultaneously detected in silenced plants and the gfp coding region of these plants was methylated extensively
在發生沉默的植株體內,在gfp蛋白及其rna被不同程度降解的同時,可以檢測到21 - 26nt的小分子rna ( sirna ) ,同時gfp基因編碼區也受到顯著地甲基化修飾。
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