major outer membrane protein 中文意思是什麼
major outer membrane protein
解釋
主要外膜蛋白-
The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。 -
Make all these together, it proved that the cloned gene represented the major outer membrane protein gene of ahl316, and the expressed gene products shared identical antigenicity with the natural main outer membrane protein. the studies on preparation and application of momp - iscoms of ah l316 provided a new approach to fish vaccinology. the successfully cloning and expressing the major outer membrane protein gene of ah l316 made it possible to describe this gene ' s function under a single factor level, and also provided technical support for developing an advanced gene engineering vaccine and subunit vaccine against aeromonas hydrophila
鰻源嗜水氣單胞菌l316主要外膜蛋白免疫刺激復合物的制備與應用研究，對研製魚類疫苗學問題進行了新的初步探索；成功地克隆和表達嗜水氣單胞菌l316主要外膜蛋白基因為在單因子水平上研究嗜水氣單胞菌外膜蛋白的作用和免疫功能以及制備嗜水氣單胞菌基因工程疫苗和亞單位疫苗奠定技術基礎。
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