melittin 中文意思是什麼
melittin
解釋
蜂毒肽-
Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin, and the sensitized fluorescent spectrometry of terbium ion, respectively. the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin. the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment, while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin. results from ft - ir spectrometry have revealed that upon binding of lanthanum ion, apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content. melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin
文摘:分別用鈣調蛋白和蜂毒素的內源熒光光譜以及鋱離子的敏化熒光光譜考察了鋱離子對鈣調蛋白構象變化以及對鈣調蛋白與蜂毒素相互作用的影響.結果表明，鋱離子首先結合在鈣調蛋白的第和第位點，鋱離子不影響鈣調蛋白與蜂毒素的相互作用，蜂毒素與鈣調蛋白作用后不影響鋱離子在鈣調蛋白上的鍵合順序.傅里葉變換紅外光譜結果表明三價的鑭離子與鈣調蛋白作用使鈣調蛋白的螺旋結構增加，折疊結構減少，與鈣離子對它的二級結構影響相類似.稀土離子在鈣調蛋白-蜂毒素復合體系中主要與鈣調蛋白作用 -
The mature region cdna of melittin of apis cerana was cloned by using puct - vector developed from pucis vector. the sequence was compared with the corresponding region of melittin of apis mellifera reported in genbank, the mutant site was found in 33rd base ( a : apis mellifera ; g : apis cerana ), but the primary structure of the two proteins were identical, which suggested melittin, as an important defensive gene, was very conservative in evolution
測序結果顯示與意蜂（ apismellifera ） melittin成熟區序列相比，中蜂（ apiscerana ） melittin成熟區序列在第33位存在差異（意蜂為a ，中蜂為g ） ，但兩者蛋白質一級結構相同，說明melittin作為蜜蜂一種重要的防禦基因在進化上保守。 -
Cationic antibacterial peptides are found in insect, animal and plant. they are grouped into four classes including cecropins, magainins, melittins and defensins. cema consists of eight amino acid from n - terminus of cecropin a and c - terminus of modified melittin
陽離子抗菌肽是一類廣泛存在於昆蟲、動物、植物體內的小分子抗菌活性多肽，大體可分為：殺菌肽（ cecropin ） 、蛙皮素（ magainin ） 、蜂毒素（ melittin ） 、防禦素（ defensin ）等四大類。 -
Megf and melittin was linked together by a linker into an chimeric dna, megfmel, and the chimeric dna was expressed by using pet30a as expression vector and e. coli as expression strain
值在slug砌l與54ug加l之間。在誘導表達各目的產物時誘導溫度為30c 。 -
This project is aimed at : ( 1 ) expressing melittin and megf in e. coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake
本課題以大腸桿菌（ e . coli ）宿主重組表達melittin和megf ；以melittin為毒性部分、 megf為靶向部分，構建膜毒性免疫毒素；用定量競爭rt - pcr法測定megf體內表達豐度；檢驗所構建的各重組菌株的遺傳穩定性；採用搖瓶分析對各重組菌株的發酵條件進行了初步優化。 -
The results indicated the spatial structure of the expressed product was correct, and the cationic region formed by two lys and two arg was necessary for keeping the activity of melittin
這些結果表明表達產物呈正確的空間折疊，而其中由2個lys和2個arg組成的正電區對melittin保持活性不可缺少。 -
Melittin, which is taken as a typical cap, has many kinds of bioactivities. mouse epidermal growth factor, megf, as a factor to stimulate cell proliferation, also has been applied widely in clinical practice
小鼠表皮生長因子（ mouseepidermalgrowthfactor ， megf ）作為一種致細胞增殖因子在臨床上也有廣泛應用。 -
Moreover we have got the macroscopic binding constants of ktb - pro by monitoring fluorescence. independent of calcium ions, protein can also combine with melittin
而且，我們從中求得了tb ~ （ 3 + ）和中心蛋白配合物的條件穩定常數。 -
Mae designed by our lab is a hybrid peptide with ideal antibacterial activity. it derives from magaininii ( amino acid residues 4 - 14 ) and melittin ( amino acid residues 2 - 13 ), and is optimized according to the relations between the structure and function
Mae是本實驗室設計的具有抗菌活性的雜合肽，它是以magainin （殘基4 - 14 ）和melittin （殘基2 - 13 ）為親本，並對序列進行了優化改造后設計出的雜合肽。
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