microfilaments 中文意思是什麼

microfilaments 解釋
微絲
  1. Polymer his - tagged peac1 - gfp efficiently activated myosin mg - atpase activity, which indicated that peacl might take part in correlative living activities in vivo. moreover, this result provided experimental proof in vitro for fusing gfp to actin isoform directly to study the dynamics of microfilaments and its regulation in vivo. we prepared rabbit anti - pea actins polyclonal antibodies using peacl as antigen which being expressed and purified from prokaryotic cells, and the antibodies possessed better immunity activity to pea actins

    通過肌動蛋白體外對dnase以及肌球蛋白atpase活性影響的研究,發現單體his - taggedpeac1 - gfp能顯著抑制dnase活性,在肌動蛋白聚合條件下能有效激活肌球蛋白atpase活性,這一結果預示著peac1在體內可能參與相關的生命活動,為利用gfp直接與肌動蛋白異型體融合來研究體內微絲的動態變化及其調節提供了實驗依據。
  2. In this study, in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo, a kind of double labeled material was constructed. the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively

    本研究以擬南芥花粉管為材料,通過轉基因技術,將分別標記ca ~ ( 2 + )和微絲的兩種熒光蛋白cameleon與gfp - mtalin在花粉管中同時表達,實現活體花粉管中ca ~ ( 2 + )與微絲的同時標記。
  3. The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition

    熒光標記結合熒光顯微觀察表明:從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察,也可被微絲的特異標記物鬼筆環肽所標記,而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致;電鏡負染的結果進一步證實其直徑為9nm ,與傳統微絲直徑相當( 7 ? 10nm ) ;聚合曲線有明顯的停滯期,為典型的s型聚合曲線,聚合臨界濃度為0 . 75 mol l ,這一結果與已有報道相似。
  4. We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants

    本研究選取正常棉花的根,已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料,採用石蠟切片,通過fitc -鬼筆環肽對材料微絲熒光染色,結合熒光顯微鏡觀察。
  5. In primitive protoctisis, as in animals, the cell membrane ingrows under the control of a ring of actin microfilaments, and a furrow constricts the cell equator until it pinches the cell in two

    在動物細胞中,胞質由肌動蛋白控制,在赤道面處向下凹溝,形成環狀縊縮,最後縊縮斷裂,細胞一分為二。
  6. Actin, the major component of the dynamic microfilaments system, exists in nearly all eukaryotic cells, and plays an essential role in living activities

    肌動蛋白在真核生物中廣泛存在,由肌動蛋白參與形成的動態微絲骨架系統是細胞生命活動的基礎。
  7. Using conventional and immunoelectron microscopic methods, the cytomixis in pollen mother cells of tobacco ( nicotiana tobacum ) and the distribution of actin microfilaments during this process were investigated in the present study

    摘要應用普通電鏡和酶聯免疫電鏡技術,研究了煙草花粉母細胞中的細胞融合現象及細胞融合過程中肌動蛋白微絲骨架的變化。
  8. After dedifferentlation and redifferentiation, the explants had three fates : abnormal plantlets, death and healthy plants. the correlation between microfilaments, actin mrna and development had been reported

    外植體通過誘導經歷脫分化和再分化,最後大致有三種去處:誘導出畸形苗,死亡和誘導出正常植株。
  9. Cytosolic free ca2 + and actin microfilaments play crucial roles in the regulation of pollen tube growth and they may interact with each other

    作為調控花粉管極性生長的重要因子, ca ~ ( 2 + )和微絲在調控花粉管極性生長過程中可能存在相互作用。
  10. Although fluorescence labeling method was widely used in the observation of microfilaments " dynamic distributions in vivo, all of the traditional methods have their limits. the use of gfp fusion and the preparation of new type of algae fluorescent probe will facilitate our further study on plant actins

    熒光標記技術雖然廣泛用於細胞內微絲骨架分佈及其動態的研究,但傳統的熒光標記方法用於植物肌動蛋白研究時都具有一定的局限性,利用gfp融合以及研製新型的藻熒光探針有助於對植物肌動蛋白的深入了解。
  11. The results showed that there was a great deal of microfilaments in root meristematic cells, there was a few microfilaments in cotton callus and abnormal plantlets ; by alignment of peac17 with cotton actin

    因此推測,微絲在細胞中的這種分佈可能與愈傷組織不能繼續誘導出正常的植株而誘導出畸形苗有關。
  12. All above showed that peacl preserved the polymerization activities of actin, and gfp fusion gave facilities for the study of microfilaments proper ties in vitro. monomer his - tagged peac1 - gfp could notably inhibit dnase i activities

    上述結果表明,通過gfp的融合而在大腸桿菌中可溶性表達的peac1 ,不僅很好保持了肌動蛋白的聚合活性,而且gfp的熒光特性也方便了微絲體外特性的研究。
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