muts 中文意思是什麼

muts 解釋
穆茨
  1. But as to muts recombinants, the periods of fermentation is too long and the efficiency of using methanol is low

    然而， mut ~ s菌株由於發酵周期長，甲醇率利用低，並不適用於實際的大規模生產。
  2. To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions

    在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。
  3. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  4. The yield reached 10 - 12mg / l. dr. chen ding hu in plant virus group of institute of plant protection of the caas ( chinese academy of agriculture sciences ) selected pap muts recombinants and wanted to produce pap through fermentation to prevention and cure plant virus disease

    中國農科院植保所植物病毒實驗室陳定虎博士成功的篩選到p . pastorispap利用甲醇緩慢型重組子（ mut ~ s ） ，希望能利用微生物發酵的方法來大量生產pap ，將其應用於植物病毒病的防治。
  5. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  6. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
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