mycoplasma hyopneumoniae 中文意思是什麼

mycoplasma hyopneumoniae 解釋
豬肺炎支原體
  1. Mycoplasma hyopneumoniae ( mhp ) is the etiologic agent of mycoplasma pneumonia in swine

    豬肺炎支原體是引起豬氣喘病的主要病原。
  2. 6 weaning pigs were immunized with membrane protein, and their antibodies were 1 : 23. 50 ( immunized after 7 days ), 1 : 26. 33 ( immunized after 14 days ) and 1 : 27. 83 ( immunized after 21 days ). humoral and cellular immunity tests testified immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein was good

    從細胞免疫和體液免疫試驗的結果可以看出,豬肺炎支原體膜蛋白具有良好的免疫原性,能誘導動物機體產生較強的細胞免疫和體液免疫應答反應。
  3. Attachment to host tissue is essential for colonization by most mucosal pathogens, such as mycoplasma hyopneumoniae. according to the reported sequence of p97 gene in strain 168, a pair of primers was designed and the rl region of p97 gene in strain 168f485 was amplicated by pcr

    本研究根據已報道的序列為參考,設計一對帶有ecor和hind酶切位點的引物,並通過引物的定點突變,利用pcr方法從豬肺炎支原體168f485株擴增到其黏附因子p97基因的抗原決定簇r1區。
  4. These essential component, hemagglutination, toxicity, immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied. the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis, and molecular weights of the protective antigens were 36kd and 64kd by sds - page

    本研究以分離豬肺炎支原體野毒株( xy - 1株)的膜蛋白為試驗材料,系統地研究了膜蛋白的分子量范圍、血凝性、毒性及免疫原性等。
  5. It was transformed into competent cells of bl21 ( de3 ), then was induced to express by 0. 2mmol / l iptg at 37 for 4 hours. the recombinant protein was nearly 23. 3 % of the total products. it was proved that the recombinant protein had immune activity through western blot and elisa analysis. this provided the basis for the future preparation of mycoplasma hyopneumoniae test kit and the new vaccine against mycoplasma hyopneumoniae

    將其轉化宿主菌bl21 ( de3 )的感受態細胞,然後經過誘導條件(誘導溫度、誘導劑用量和誘導時間)的優化,結果發現在37下, iptg終濃度為0 . 2mmol l ,誘導4h進行表達,可得到了一分子量約265kda的融合蛋白,表達量可達約23 . 3 。
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