n-terminus 中文意思是什麼

n-terminus 解釋
n末端
  • n : 1. 【羅馬數字】90〈N=90000〉。2. 【化學】=nitrogen. 3. =North(ern)。N =nuclear 核的:N-waste 核廢料。
  • terminus : n (pl terminuses ni)1 (鐵路,汽車,航路等的)終點。2 〈英國〉終點站〈美國叫 terminal〉。3 界限...
  1. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因:其全序列共1614bp (從起始密碼子atg到s前體蛋白裂解位點) ,編碼537個氨基酸,其氨基端有一編碼18個氨基酸的信號肽序列,第12 13位氨基酸殘基構成了信號肽的切割位點, 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  2. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有( his ) 6特點,將融合蛋白進行純化,在純化過程中經各項條件的探索,確定為在裂解液中含有1mmpmsf的條件下,分別經過2倍體積的buffera和bufferb洗脫后,再收集ph5 . 9 ,含有80mmol / l咪唑的1倍體積bufferc洗脫液,可得到純化的融合蛋白。
  3. In escherichia coli, arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway. here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog, and the effects of n - terminus on feedback inhibition and its quaternary structure, and the importance of the structural " d2 " symmetry to allosteric inhibition

    本博士論文工作以大腸桿菌k - 12來源的arog為研究對象,通過定點突變、反饋抑制實驗和酶學動力學參數的測定,深入地研究了arog的反饋抑制位點的特性,並對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用,以及蛋白質結構的「 d2 」對稱性對酶功能的重要性等進行了具體的研究。
  4. To investigate the secondary structure of this gene, we found it has a transmembrane region near the n - terminus, followed by a proline - rich conserved region, and it has a conserved haemachrome binding region about 60 aa away from carboxyl terminus

    分析氨基酸序列的二級結構發現基因n -端具有跨膜的疏水序列,其後具有富含脯氨酸保守區,在距離羧基端60個氨基酸處具有高度保守的血紅素結合域。
  5. The ir sequence of mxmybl is most homologous with that of atmyb ( 86. 9 % ) and somewhat homologous with that of stmyb 1 ( 77. 0 % ) ; there is very low homology among n - and c - terminal regions outside of the ir regions of all of the mybs ; the protein mxmybl contains a proline - rich region as well as a serine - rich region near the c - terminus, such structure motifs are implicated in transcriptional activation

    9 ,與馬鈴薯stmyb的ir序列的同源性達77 0 ,所有這些nnrb蛋白除了瓜區具有較高的同源性外,其c端和n端幾乎沒有同源性。 mwyb蛋白的c一端還含有一個富含脯氨酸區,這樣的結構基序可能具有激活轉錄的功能。
  6. In this paper, signal peptide was added before the n - terminus of cema with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity

    本文通過在cema的n端添加信號肽,對其進行改造,以探索不降低抑菌作用的同時,降低其對植物細胞毒害作用的可能性。
  7. On the other hand, our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells. the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells

    我們以往的研究表明,用綠熒光蛋白( gf內在信號膚下游n末端標記nrzb亞單位,與nri共轉染時能形成與野生型相似的nmda受體通道,且可以通過抗gfp抗體標記活細胞膜表面表達的nmda受體復合物。
  8. Cationic antibacterial peptides are found in insect, animal and plant. they are grouped into four classes including cecropins, magainins, melittins and defensins. cema consists of eight amino acid from n - terminus of cecropin a and c - terminus of modified melittin

    陽離子抗菌肽是一類廣泛存在於昆蟲、動物、植物體內的小分子抗菌活性多肽,大體可分為:殺菌肽( cecropin ) 、蛙皮素( magainin ) 、蜂毒素( melittin ) 、防禦素( defensin )等四大類。
  9. Leung king shopping centre, leung king estate, tuen mun, n. t. opposite to the bus terminus

    新界屯門良景良景商場巴士總站便利店對面
  10. The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs. the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs. the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide, two krab domains and 14 c - terminal c2h2 zinc finger motifs

    Znf325長2697個堿基,含有一個長為1389個堿基的開放閱讀框,編碼一個長為462個氨基酸的蛋白質,它包含15個c2h2型鋅指結構; znf359長3270個堿基,含有一個長為1932個堿基的開放閱讀框,編碼一個長為643個氨基酸的蛋白質,它包含一個n -端krab結構域和16個c -端c2h2型鋅指結構; zfp28長4104個堿基,含有一個長為2076個堿基的開放閱讀框,編碼一個長為868個氨基酸的蛋白質,它包含一個n -端信號肽,兩個krab結構域和14個c -端c2h2型鋅指結構。
  11. Not only sequence information from the c - terminus of proteins and peptides is of especial interest in the investigation of n - terminally blocked proteins and peptides, but also c - terminal sequence analysis can facilitate the production of more specific probes for gene cloning

    C端與n端一樣,在蛋白質分子結構分析中具有重要的地位,對其順序測定具有重要的意義,不僅可以對n端封閉的蛋白質進行序列測定,而且對基因克隆有指導意義。
  12. The complete human znf359 cdna sequence is 3270 bp and contains a 1932 bp open reading frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs

    Znf359基因長3271個堿基,含6個外顯子,在基因組dna上長18kb 。它編碼的蛋白質全長643個氨基酸,含一個krab框, 16個c2h2型的鋅指,在兩者之間還有一個典型的中間重復序列。
  13. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵,包括36個氨基酸組成的疏水信號膚,一個cub ( clr / cls , anembryonicseaurehinproteinuegf , andbonemorphogeneticproteinl )結構域和一個胰酶樣絲氨酸蛋白酶結構域( t汀psin一likeserineproteasedomain )和幾個保守的糖基化位點等,沒有發現有跨膜區的存在。
  14. The n - terminus truncated mutant can not form tight dimeric and tetrameric structure, and leads to the destruction of the quaternary structure, which is quite the contrary to wild type arog. these data indicate that n - terminus of arog is not only involved in the feedback inhibition, but also plays a role in the formation of stable dimer which is an essential structure for its enzymatic activity. according to the 3d structure, " d2 " symmetry of arog, to which contributed by leu 16, trp215 and his217, is essential for the feedback inhibition

    對「 dz 」對稱性相關的氨基酸leul6 、 trpz15和hisz17進行點突變和雙突變研究結果表明: l )在1mm苯丙氨酸的存在下, leul6ala 、 trpz15ala和hisz17ala能解除35一70 %的反饋抑制, t印2巧ala的比活降低65 %以上,其餘兩者的比活與野生型相近; 2 )在lmm苯丙氨酸的存在下,導入雙點突變trpz1sai洲isz17ala和leul6hi撇isz17leu后能夠完全解除arog的反饋抑制,同時保持與野生型相當的比活。
  15. Sequencing and genbank blasting demonstrated that these four had the same sequences and encoded part of arabidopsis choloroplast carbonic anhydrase ( ca ). the coding region contained 251 amino acid, from n terminal amino acid 86 to c terminus

    到genbank進行序列比對,結果表明它們編碼擬南芥葉綠體碳酸酐酶( ca )基因,編碼區含有部分ca序列,從n端第86位氨基酸至c末端,共251個氨基酸。
  16. It encodes a protein of 865 amino acids with a signal peptide at the n - terminus, of which a polycystic - kidney - disease ( pkd ) - like domain, a triosephosphate - isomerase ( tim ) catalytic region, a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus

    根據已知幾丁質酶基因的dna保守序列,利用人工合成的pcr引物和cb101總dna ,擴增出一個約2 . 6kb的片段並克隆到pbluescript ks載體上。
  17. The orf1 protein of senv - h12 - 1 isolate was consisted of 675 amino acids and 92 amino - terminal amino acids less than senv - h ( ax025838 ), it had insertion in two sites also. the orf1 protein of senv - h 12 - 2 isolate was 7 amino acids less than senv - h ( ax025838 ) because of deletion. because the first start code was mutated, the orf2 protein of senv - h 12 - 2 isolate started from the second atg code, so that it was 7 amino acids less than senv - h ( ax025838 ) from its n - terminus

    兩個senv - h亞型分離株的情況與已發表的博士學位論文: sen病毒分子病毒學和流行病學研究對sen琴h ( ax025838 )株分析的結果有所不同: senv - h12一1分離株的orfi僅由675個氨基酸組成,比senv - h ( axo25838 )的orfi少n一末端的92個氨基酸,此外在2個位點上還存在插入: senv - h12一2分離株在一些位點上的缺失造成o盯1蛋白比senv - h ( axo25838 )少7個氨基酸,由於第一個起始密碼子發生突變,採用位於下游的第二個起始密碼子使得o即2蛋白比senv - - h ( axo25838 )少n一末端的7個氨基酸。
  18. The amino acid analyzer could n ' t detect amino acid sequence with edman degradation, which meant apss had the blocked n - terminus

    N一末端測序法不能分析apsi和」 s5氨基酸序列,估計其n一末端封閉。
  19. We obtained two positive clones after screening. sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis. the coding region was from n terminal amino acid 240 to c terminus

    測序及序列比對結果表明二者序列相同,為ked樣蛋白的部分編碼序列,編碼部分從n端第240位氨宋林夜:利用酵母雙雜交系統篩選與g蛋白及cam相互作用的新型蛋白基酸至c末端,共207個氨基酸。
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