negative strain 中文意思是什麼

Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly. 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so

3n ） ，將待測eaggec菌株的培養上清加入該報告菌株培養物中，用流式細胞術facs方法檢測報告菌株熒光強度的變化情況，間接反映ybt的表達與否。

The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。

. moreover, some samples appeared several active bands in the gel, which indicated the existence of different types of sod or multi - subunits of sod in these samples. the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella, which observed after the gram ' s staining and flagellum staining

同時，利用非變性聚丙烯酰胺凝膠（ page ）電泳后的凝膠顯色反應，發現一些樣品出現了多條活性帶，這可能是因為在這些細菌提取物樣品中含有不同類型的sod分子，或是同一類型的sod含有多個亞基組成。

The strain and its metabolites are identified. the results show that the culture has a broad antimicrobial spectrum against some gram - negative and gram - positive bacteria, and moulds, especially against e. coli, bacillus cereus, and staphylococcus aureus

研究證實，分離出的r21 - 4具有廣譜抗菌作用，對革蘭氏陰性菌、革蘭氏陽性菌、真菌都有一定的拮抗作用，特別是對大腸桿菌、金黃色葡萄球菌、蠟狀芽孢桿菌抗菌作用較強。

Based on the results of testing site, some problems of vacuum preloading was analyzed by plane strain finite element method. the relative stable seepage velocity field can be produced with the pumping time. the depth of negative pore - water pressure transferring increases with the pvds length

最後，對真空聯合堆載預壓的施工工藝進行了研究，提出了在真空聯合堆載預壓的中期，出水量已相對較少，可適當減少開泵量，並可採用停抽結合的變真空能量法，在不影響加固效果的前提下，可以大大節省用電量。

5. the morphology of the two strains also shows that the flagella of ss - ori are around the cells, whilst the strain yz - ii6 has only a single flagellum grown at the polar of cells besides the different of cell size, though they both are bacillus and gram - negative

形態和生理生化特徵也表明， ss - ori菌周生鞭毛，革蘭氏反應呈陰性， yz - 6菌為極生單根鞭毛，革蘭氏反應呈陰性。

Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。

Based on koji making and fermentation with multi - strain or single - strain, the result showed that multi - strain was not suitable to and had negative effect on soy sauce production

摘要通過一系列的多菌種和單菌種在相同條件下制曲發酵對比實驗，證明多菌種制曲發酵不但沒有益處，而且有明顯的負作用。

Test results show that : ( 1 ) the amount of creep deformation during monotonic primary loading depends on strain rate before creep, creep stress level, and creep time ; ( 2 ) during monotonic primary loading, the stiffness increasing magnificently shortly after creep ; ( 3 ) the direction of creep will change from positive creep ; to neutral creep and negative creep during unloading, while during reloading, it will change from negative, to neutral and positive creep

試驗結果表明： （ 1 ）在初始加載過程中，經過一定時間蠕變后黏土的強度有很大的提高； （ 2 ）在卸載過程中，隨應力值下降黏土的蠕變表現出由正蠕變、中性蠕變到負蠕變的逐漸轉化； （ 3 ）在重復加載過程中，隨應力值增加黏土的蠕變表現出由負蠕變、中性蠕變到正蠕變的逐漸轉化。

The very plasmid pbmb0474 was constructed. when the recombinant plasmid pbmb0474 was transferred into crystal negative bt strain bmb171 through electroporation, the expression of the fusion genes about 150kda - 160kda can be detected by sds - page. 4

將pbmb0474電轉化到bmb171中得到蘇雲金芽胞桿菌重組菌， sds - page結果顯示重組菌可以表達大小約150kda - 160kda的gfp融合蛋白。

The results show that there are 7 strains hpi positive except 1 strain hpi negative. hpi in 6 strains of 7 hpi - positive strains is inserted into asnt - trna site. the expression of fyua gene, which encoded fyua, the receptor of ybt, was upregulated by extracellular ybt level

研究結果提示，除1株eaggec中國分離株hpi 」外，其餘7株均為hpi且7株中有6株攜帶的hpi毒力島均插入在染色體的asnttrna位點。

4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition

解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。

Research on e. coli protease system is the basis of engineering strain improvement in order to reduce their negative effect, much has been done, among which antisense rna technology is worth further trying

為減少蛋白酶對外源蛋白的降解，從不同的角度做了大量工作，其中利用反義rna技術控制蛋白酶活性是一條值得探索的思路。

( 2 ) because of t he variability of rock subjected to outside action, three new conceptions : postive damage basic damage and negative damage, are suggested, which can deal with the condition that rock density became large. ( 3 ) the present damage variables based on ct number mostly include ct average number of the rock without damage, which is difficult to measure, so a new damage variable, which includes the average ct number is proposed, on the other hand, strain equal theory is generalized because the one put foreword by lemaitre is not good enough to use. ( 4 ) a axial damage constitutive equation is proposed and testified. ( 5 ) with the circle times, the damage propagation laws of two different rocks, subjected to circle of freezing and thawing, are studied, and in the analysis process, traditional analysis method including average ct number and is used, some important conclusions are reached : inner damage of soft rock containing a large mount of apertures is largely propagating with the time increasement of circle, however, at the preliminary time, the hard rock containing a small mount of aperture is a little propagating, at late time, the influence become large ; ( 6 ) zone separation and statistics frequency put forward is used to analysized the three different damage propagation in the open circumstance, when they are subjected to the different circle times of freezing and thawing, in which the temperature variability is linear, different freezing rate, and different freezing temperature, and no water is supplied during the test

在研究過程中本文主要做了以下工作： （ 1 ）針對巖石損傷變化較小時，運用ct均值和方差分析不方便的缺陷，本文提出運用對ct數范圍劃分區段的方法，根據各區段統計頻率的變化規律來分析損傷擴展狀況； （ 2 ）針對巖石受到外部作用的多變性，本文提出了基準損傷，正損傷，負損傷的概念，在某種程度上考慮了巖石受到外部作用時的壓密情況； （ 3 ）現有的基於ct數的損傷變量大多需要用到巖石基體的ct均值，這實際上是很難觀測到的，因此本文建議了一個可運用巖石初始狀態ct均值的損傷變量，由於現有的勒邁特教授應變等價原理使用上的不便，本文在其基礎上進行了推廣； （ 4 ）建議了一個可用巖石初始狀態ct數和彈性模量的單軸損傷本構方程，並用算例進行了驗證； （ 5 ）對兩種不同巖石在開放環境下受到凍融循環作用且融化過程中補水時，內部損傷隨凍融循環次數增加的擴展規律進行了探討，主要運用了常規的ct均值和ct方差的分析方法進行分析，試驗發現：對于孔隙率和含水率較高，密度和強度較低的軟弱巖石，凍融循環次數對損傷結構的擴展有明顯的影響，而對于孔隙率和含水率較低，密度和強度較高的硬巖，凍融循環次數對其損傷結構擴展初期由於含水率低的原因，影響不是太大，而後期由於水補給后，含水率較高的原因，凍融影響逐漸增大； （ 6 ）運用了本文提出的區段劃分和統計頻率的方法，深入地分析了開放環境下的線性溫度變化的凍融循環次數，凍結溫度，凍結速度不同巖石作用時，其內部損傷隨凍融循環次數，凍結速度和凍結溫度變化的擴展規律，需要指出的是除了在試驗前飽水外，凍融過程中沒有補水。

We analyes post - tensioned prestressing properties and comparing with practiced results. we analyse one - way precast slab sandwich negative moment section resistant crack properties and two - way precast slabs sandwich under plane resistant crack at unprestressed section and second - prestressing propties. we compare canceling bonded reinforcement with laying on bonded reinforcement resistant crack properties and deformation. auxiliary lab include : concrete resistant pollution intensity. concrete resistant compressing intensity and elastic modulus, compare with results of code. analyse sandwich ' s strain and long - time stress, strain and introduce a new method through theory calculating long - time stress and strain

配套的試驗研究有：混凝土的抗拉強度和彈性模量試驗及混凝土的抗壓強度和彈性模量試驗，混凝土的極限抗拉強度和極限拉伸應變試驗，並與《混凝土結構規范》 （ gb50010 - 2002 ） ，結果進行了對比；單塊夾層板放張階段的彈性應力、應變分析和長期應力、應變分析，並詳細介紹了按混凝土徐變理論進行長期應力、應變分析的方法。

In the study, respiratory problems and blue ear were not observed on all the pigs after challenged with prrsv strain ch - 1a. but the rectal temperatures of the pigs in the negative control groups ( plers1neo - immunized group and mock group ) were higher than the pigs immunized with the dna vaccines

Prrsv的感染一般為亞臨床表現，在本試驗中，攻毒后所有的小豬都沒有出現呼吸困難、耳朵發蘭等癥狀，但空白對照豬和空質粒接種豬的體溫要高於基因疫苗免疫豬和滅活苗免疫豬，最高可達40石c 。

Kustaki : ori44, oriw30 and ori2062 were chosen to construct the gfp resolution vector. there were 9 kinds of gfp resolution vector. all of the recombinant plasmids were introduced into crystal negative b. thuringiensis 8mb 171. the fluorescence of the 9 kinds engineered strain can be detected by the fluorescent microscope

將得到的9種gfp解離載體電轉化到蘇雲金芽胞桿菌無晶體突變株bmb171中，熒光顯微鏡鏡檢顯示9株含gfp的蘇雲金芽胞桿菌重組菌在波長為300nm - 510nm的激發光下可發射綠色熒光。