nisa 中文意思是什麼
nisa
解釋
萊塞-
Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production
以lactococcuslactisnz9800為受體菌,研究了表達載體的表達情況。 -
4. the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800. sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production
抽提ecolijm109中的重組質粒,轉化l . lactisnz9800 ,在含紅黴素的gm17平板上篩選轉化子。 -
3. the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e. the ligation mixture was transformed into jm109 for the initial cloning. the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion
將酶切並純化后的pcr擴增產物與大片段連接並轉化e . colijm109 ,在含紅黴素的lb平板上篩選到含有重組質粒的轉化子。
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