northern blot hybridization 中文意思是什麼

northern blot hybridization 解釋
印跡雜交
  • northern : adj （superl northernmost） 北的，北方的，住在北部的；北方特有的；〈N 〉美國北部的。n 1 北方人 （...
  • blot : n 1 墨污，墨漬，污點，污斑。2 瑕疵；恥辱，污名。3 〈古語〉塗去，抹去。vt （ tt ）1 用（墨水等）弄...
  • hybridization : 混成作用
  1. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  2. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  3. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ e
  4. Northern blot hybridization showed that leacbf expression had the same tendency with the enzyme activity. and it showed that the transcription factor acbf maybe have some relation with the enzyme activity of pal

    表明在紫外光照射條件下， leacbf編碼的轉錄因子的變化趨勢與苯丙氨酸解氨酶（ pal ）活性的變化趨勢有一定的相關性。
  5. Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ). treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased

    用不同的紫外線照射時間處理番茄幼芽，苯丙氨酸解氨酶（ pal ）的活性呈先是增加，達到最大值后再減小的趨勢。
  6. Northern blot hybridization showed that leerfl, leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening, which was related with fruit ripening

    Northern雜交分析表明： leerf1 、 leerf2 、 pti4 （已發表的番茄erebps成員）基因在普通番茄果實的成熟過程及果實的不同部位都有差異表達，表現出與成熟進程的相關性。
  7. We further performed northern blot hybridization to reveal the regional distribution of 3galt - l transcripts in mouse brain. in cerebral cortex, p3galt - l was expressed abundantly in newborn mice with a slight decrease in adult mice, and a similar expression pattern could also be detected in hippocampus

    我們發現：在大腦皮質和海馬中， 3galt - 1的表達水平在發育進程中都相對較高；在間腦和腦干中， 3galt - 1的表達水平則較低；而在小腦中， 3galt - 1在新生鼠中表達很高而在成年鼠中表達明顯下降。
  8. 61 of these clones have hybridization signals on the x - photos of the reverse northern blot. then northern blots were performed for 23 of these 61 clones : nos. 4, 17, 18, 23, 28, 66 - 1, 69, 84, 89 - 1, 94, 97, 101, 108, 152, 156 - 1, 157 - 1, 165, 172, 175, 183, 185, 191 and 233

    其結構功能域中包含1個28個氨基酸殘基的信號肽ol2個晚期胚胎發生高豐度蛋白lea毛結構域（ 56 206 ， 205 323 ） 、 2個保守分泌蛋白cog5608結構域（ 72 200 ， 174 281 ）和3個低復雜度區門， 4265 ， 329習44其h級結構中存在有大量的捲曲螺旋區。
  9. In order to assess the function of kyot2, northern blot, immunohistochemical sabc and in situ hybridization methods were used to find out the expression of kyot and kyot knockout mice which were kept in our laboratory were analyzed. moreover, we employed a yeast two - hybrid screening using kyot2 as a bait protein and got 13 interacting proteins. the function of a novel protein of them, kbp was investigated

    為了進一步研究kyot的功能，我們利用northern印跡、兔疫組織化學等方法對kyot在體內的分佈進行時空定位，並利用本室懈的kyot基困剔除小鼠分析其表型，同時採用酵母雙雜交篩到tkyoth相互作用的13種分子，並對其中的一個新分子kbp進行了功能的研究
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