nucleotide pair 中文意思是什麼

nucleotide pair 解釋
核苷酸對
  • nucleotide : n. 【生物化學】核苷酸。
  • pair : n （pl pair（s））1 一對，一雙，一套，（眼鏡等的）一副；（剪子等的）一把，（褲子等的）一條。 2 一...
  1. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  2. According to the reported complete nucleotide sequence of goose parvovirus in genbank. with oligo4. 1. a pair of primers were designed and synthesized

    與主要結構蛋白vp3存在相同的抗原決定簇成分，是制備基因工程疫苗的重要候選基因。
  3. Culture supernatants of bt strains induced luminescence in v. harveyi reporter strain bb170, which can only response to ai - 2 signal moleculars, indicating that the bt strains have the luxs gene. by aligning the nucleotide sequences of the b. anthracis and b. cereus luxs genes, a pair of primers were designed and an 474 - bp fragment was amplified from bt strains by pcr

    首先通過生物發光實驗檢測到蘇雲金芽孢桿菌（ bacillusthuringiensis ， bt ）的培養液可以誘導特異性檢測ai - 2信號分子的哈氏弧菌報告菌株bb170發光，表明bt中含有群體感應信號分子ai - 2的合成基因luxs 。
  4. A pair of primer, dig labeled probe and a taqman probe based on the conserved nucleotide sequence of cp gene of different pnrsv strains were designed and synthesized

    本文根據病毒各株系外殼蛋白基因的保守序列，設計了雜交誘捕探針、 dig標記雜交探針以及確定了最佳誘捕參數和雜交檢測參數，建立雜交誘捕rt - pcr ? elisa 。
  5. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  6. The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1

    本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。
  7. Complete co i sequence, co ii sequence and co i + ii sequence were used for comparison. nucleotide composition and variation of each sequence fragment were analyzed using mega2. 1. genetic relationships among haplogenotypes were estimated based on the pair - wise matrix of sequence divergences by kimura ' s two - parameter

    第1180一1569位區段分子樹表明黃紋鱗石蛾與弓突鱗石蛾的親緣關系較近，與根據形態學建立的系統關系相吻合。
  8. According to the reported complete vp6 nucleotide sequences of group a prv in genbank, a pair of primers was designed to amplify vp6 gene of jl94 with oligo 4. 1 software. the segments of complete gene 6 of jl94 were obtained from rt - pcr using dsrna extracted from the virus as template

    根據genbank中的多株豬a群輪狀病毒vp6蛋白完整基因序列，利用oligo4 . 1設計引物，以jl94rna為模板，通過rt - pcr擴增出長約1 . 3kb的基因片段，命名為vp6 。
  9. By rt - pcr method. we first selected a pair of primers named 2bp and 4bm which was designed in highly conserved 922 nucleotide region in open reading fram ( orf ) ib from coronavirus. this primer could amplify 11 kinds of coronaviruses. after synthesizing this pair of primers, we amplified the target fragement of 251bp from the panda ' s liver - tissue materials and other different passages of culture of this virus. however, the control cell showed negative result

    第一對引物為外層引物，可擴增出1086bp的核酸片段；第二對引物為內層引物，可擴增出515bp的核酸片段。該引物以k378為參考株，含有多個兼并堿基，可擴增出包括k378 、 insavc - 1 、 ccv1 - 71 、 nvsl吉林農業大學碩士學位論文大熊貓犬冠狀病毒的分離鑒定及s墓因部分序列的測定和分析等多種犬冠狀病毒。
  10. Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3

    各另外設計一對特異性pcr引物，導入適當限制性內切酶切點，以上述連有目的基因的克隆載體為模板，採用pcr方法擴增基因片段，獲得長度約230bp的igf -和219bp的igf -成熟肽基因序列。
  11. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
  12. In this paper, we analysis the information of nucleotide or amino acid sequences with computer technology and finally give a computation result which is an alignment of either pair - wise sequences or multiple sequences of nucleotide acid or amino acid

    本文通過計算機技術，對于核苷酸或氨基酸中的信息進行分析，針對兩個或者兩個以上的核苷酸序列或氨基酸序列，經過計算，最終給出比對結果。
  13. In order to study mhc genes of chinese a igator ( alligator sinensis ), a pair of degenerate primers were used to amplify the segments of mhc class ii b genes from the genomic dna of chinese alligator. ten 166 bp ( one 160bp ) - long different nucleotide sequences, which were divided into two groups ( a, b ), were obtained from cloning and sequencing

    為了研究揚子鱷（ alligatorsinensis ）的mhc基因，本文利用一對簡並引物擴增出揚子鱷mhc類b基因第二外元的部分片段，並對其進行了克隆及序列分析，結果得出10種不同序列，片段長166bp （其中一種為160bp ） 。
  14. To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121

    為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。
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