nucleotide sequence homology 中文意思是什麼

nucleotide sequence homology 解釋
核苷酸序列同一性
  • nucleotide : n. 【生物化學】核苷酸。
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  • homology : n. 1. 相應，符合；關系相同。2. 【化學】同系（現象）。3. 【生物學】同源。4. 【數學】透射；同調。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned

    從陽性克隆子中提取克隆片段，經序列測定分析，結果表明，克隆片段的cdna大小為816bp ，編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank ， embl ， ddbj ， pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索，結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ；蛋白質氨基酸序列的同源率為90 95 ，表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4
  3. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  4. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  5. The cloned segment a contains 3259 nucleotides in full - length and includes two partially overlapping open reading fragments ( orf1 and orf2 ) flanked by 5 " and 3 " noncoding regions ( ncr ). the two strains shared high sequence homology with each other either at nucleotide or deduced amino acid level

    序列測定結果表明，兩株病毒的基因組a節段全長共3259個核苷酸，包括5 』 、 3 』端的非編碼區（ ncr ）和兩個部分重疊的開放閱讀框（ orf1和orf2 ） 。
  6. Due to high homology of nucleotide between ba - dfe and subtilisin bpn, primers were designed and synthesized. the intact ba - dfe gene was amplified by pcr and cloned. the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp, which encodes 382 amino acid residues containing signal peptide, pro - peptide and mature peptide

    序列分析顯示，該片段的核昔酸序列中含有1146hp的開放閱讀框，可編碼382個氨基酸殘基的ba dfe前體蛋白，包括30個氨基酸殘基組成的信號肽、 77個氨基酸組成的前導肽和275個氨基酸殘基組成的成熟肽。
  7. The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi

    與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。
  8. According to the southern hybridization results, 43 bacillus strains were divided into several groups, 22 strains were chosen randomly from all these groups for sequencing of aii gene. it was found that the nucleotide acid sequence of aii genes showed 85. 4 % - 100 % homology and amino acid sequence of aii proteins showed 8 8. 1 % - 100 % ho mology

    在southern雜交的基礎上，將檢測的43個菌株分組，對其中22個菌株的aii基因進行dna序列測定，結果表明，不同菌株aii基因的dna序列同源性為85 . 4 ? 100 ；而aii蛋白的氨基酸序列同源性為88 . 1 ? 100 。
  9. Chicken tyrp1 exons show 70. 1 % nucleotide sequence homology with human exons. fish result shows that tyrp1 locates on the long arm of z and w near to the centromere

    用熒光原位雜交進行tyrp1的染色體定位，首次將tyrp1定位在z和w染色體長臂近著絲粒的位置。
  10. The special primers were designed and synthesized after the analysis to the nucleotide sequence homology of etrl recorded in genbank. the total rna extracted from latex was reverse - transcribed into first strain of cdna. the special amplification products ( cdna fragment ) were obtained from pcr when using the first strain of cdna as template

    通過對genbank登錄的已克隆的植物etr1基因序列進行核苷酸同源性分析，設計特異引物。以膠乳總rna為模板，反轉錄成cdna第一鏈，又以cdna第一鏈為模板進行pcr擴增，得到特異擴增片段。
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