oligo-dt 中文意思是什麼
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Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3 - dctp or cy5 - dctp for the experimental and control group respectively. the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h
提取as _ 2o _ 3作用k562細胞前後的總rna ，用superscript逆轉錄酶逆轉錄成cdna第一鏈，並在逆轉錄的過程中，用cy3 cy5熒光染料分別標記對照組處理組，與自製的k562細胞基因表達譜晶元雜交。 -
Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本試驗的目的旨在進行hypodeminc （ hc ）和hypodermina （ ha ）基因的克隆、測序、構建重組表達載體並誘導表達，獲得重組抗原，以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。 -
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。
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