oligonucleotide 中文意思是什麼

Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0

寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。

To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。

Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays

計算用於微陣列的基因表達譜、和基因分型研究技術平臺使用的、低聚核苷酸探針裝置的溶解溫度分佈。

A colorimetric silver oligonucleotide arrays for detecting and identifying of pathogenic microorganisms

納米金比色晶元檢測和鑒定病原微生物

The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide, and some factors affect immobile ratio of oligonucleotide probe, such as aminosilane treatment time, aldehyde treatment time, uv crosslinking energy, washing temperature and time

研究表明，氨基化試劑濃度對玻片熒光背景有影響，氨基化試劑處理時間、醛基化處理時間、紫外交聯能量和洗滌溫度和時間等工藝因素影響寡核苷酸探針的固定率。

First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。

It was proved that tle1 expression was up - regulated in cervix cancer, while aes expression was up - regulated in gastric cancer. antisense oligonucleotide or small interfering rna specific against aes or tle1 could inhibit the expression of corresponding molecule

設計、合成針對這兩種分子的反義核酸和小分子于擾rna （ sirna ） ，轉染宮頸癌細胞系hela和胃癌細胞系sgc7901 ，發現反義核酸和sirna均可抑制相亡分子的表達， sinya的抑製作日更為明顯。

Effects of antisense oligonucleotide on expression of tissue factor in culturec human umbilical vein endothelial cells injured by anoxia - reoxygention

反義寡脫氧核苷酸對內皮細胞缺氧再復氧損傷組織因子表達的影響

Reliable detection of rifampin - resistance of mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray

檢測耐利福平結核分枝桿菌寡核苷酸晶元的研製及應用

The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide. the more oligonucleotide probes have been linked with active group, the stronger fluorescence intensity is. for the strongest fluorescence, the technical conditions is : treatment of 2 % aminosilane of 20 minutes, treatment of 5 % aldehyde of 24 minutes, uv crosslinking of 150mj and washing of 5 minutes at 20

兩種檢測方法表明，當活性基團呈柱狀、分佈均勻且尺寸比較大（ 200nm ）時，有利於寡核苷酸探針的連接，且連接探針數量多，玻片表面熒光強度強，固定率高；當活性基團呈錐狀、分佈及尺寸不均勻（ 150nm （ 300nm ）時，連接的寡核苷酸探針數量少，玻片表面熒光強度弱，固定率低。

5. prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate, meantime, add p2, low homologic probes and probe buffer in the same concentration as positive control, blank control and negative control respectively. spot microarray as designed by the spotting machine

5 .寡核昔酸微陣列的制備以同等濃度的反義鏈引物咫作為陽性對照，以探針緩沖液作為空白對照，以無關探針作為陰性對照，與制備好的16條寡核昔酸探針，同時加人384孔板。

Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。

Sixty 10 - oligonucleotide primers from opron inc. were used for polymorphic selection, with fifty four ( 90 % ) primers that produced polymorphic products. a total of 209 bands amplified from 20 primers were selected for rapd analysis

對60個來自opron公司的十堿基隨機引物進行了多態性篩選，其中54個00能擴增出多態性譜帶。

From a cross 18 x 28 ( each from the superior tree from xianju county and pan " an county in zhejiang province ). 236 random oligonucleotide primers were screened and 33 primers were selected to generate rapd markers within a sample of 60 megagametophyte dnas

通過236個10bp的隨機引物或組合對6粒黃山松種子胚乳組織dna進行pcr擴增，共篩選出33個多態引物用於作圖。

Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。

Co - culture of sperm with endometrial cells treated with antisense oligonucleotide against cftr, or with bicarbonate secretion - defective cf epithelial cells, resulted in lower sperm capacitation and egg - fertilizing ability

相反，如果用缺乏cftr的細胞，或者是cftr發生突變的細胞，精子便不能有效獲能。

To improve the performance of assays on the oligonucleotide microarray, the factors that influence the hybridization effects such as surface chemistry, probe size, spacer length, hybridization conditions etc were intensely studied and optimized

為改善寡核苷酸晶元的分析性能，對影響晶元雜交結果的因素，如片基表面的化學處理、探針的長度、間隔臂的長度、雜交條件等，進行了深入的研究和優化。

We had estimated the specificity and sensitivity of two nested oligonucleotide primer pairs. the sensitivity of these primer sets was loocopies. in order to investigate the prevalence of senv - d and senv - h in china, some kinds of hepatitis patients serum and blood donors serum were collected and tested

此外我們的調查發現senv - d舊在non一a一e肝炎患者中的感染率也顯著高於獻血者和甲肝患者（ 68 % vs31一36 % ， p < 0 . 01 ） ，而與其它肝炎患者中的感染率相當。

Preliminary study on hla - dqa genotyping by unmodified - oligonucleotide microarrays

分型的初步研究

In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。