open gene 中文意思是什麼

open gene 解釋
開放基因
  • open : adj 1 開著的，開放的；可進入的，可分享的 （to）; 無蓋的，敞口的；敞開的；展開的；開的；開闊的，開...
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The world ' s oldest film festival prides itself on promoting cutting - edge and innovative movies but this stunning lagoon city also welcomes with open arms hollywood superstars like gene hackman and nicole kidman

    不過這座美麗的水城也熱情歡迎簡-哈克曼和尼柯爾-基德曼等好萊塢巨星的到來。
  3. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  4. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  5. Some open testing datasets and real gene microarray data are applied in experiments. the results have verified the feasibility and validity of the pica to get the gene microarray data and the advanced boosting algorithm for gene classification

    經過對公開的測試數據集和真實的基因微陣列數據大量實驗，證明了用部分獨立分量分析方法獲取基因微陣列數據及boosting改進演算法進行基因模式分類的可行性和有效性，最終也完成了基因模式的識別任務。
  6. Badh gene extends 7. 7kb containing 15 exons with the open reading frame of sooaa, 1. 2kb putative promoter region

    該基因全長7774hp ，包含15個外顯子， 14個內含子以及約1
  7. The results of sequencing showed that jl94 isolate complete gene 6 was 1356bp and had a complete open reading frame which encoded 397 amino acides

    對克隆的vp6基因進行序列測定，測序結果顯示jl94vp6基因全長1356bp ，含有完整的開放閱讀框架，編碼397個氨基酸。
  8. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  9. The sequence 56 analysis shows that there is a long open reading frame in it which encode a protein of 364 animo acid residues. the m protein include 47 basic amino acid, 30 acidic amino acid, which cause a basic estimated pi of 9. 7. phylogenetic tree based on ndv matrix protein gene shows that the b95 and v4 has the most close relationship than other ndv strain

    關于ndvm蛋白國內未見有報道，國外對此蛋白的研究也十分有限，本文克隆出了ndv株b95m蛋白基因的全序列，為m蛋白的表達及進一步研究其在病毒復制與致病中的作用機理打下了基礎。
  10. Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame

    人類gnt - v由741個氨基酸組成，有6個潛在的n -糖基化位點，基因定位於染色體2q21 ，含有17個外顯子，其開放閱讀框架由外顯子2 - 17進行編碼。
  11. The open reading frame of the ibee gene is 1383bp encoding a 50kd hypothetical protein

    （五） ibeb蛋白亞細胞定位的研究1 .重組質粒pr
  12. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  13. Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression

    Pcr產物經純化、酶切后，按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶（ gst ）基因的下游。
  14. The entire halcs gene is located on a megaplasmid and contains a 849 - bp open reading frame that encodes a polypeptide of 283 amino acids. the promoter is typically haloarchaeal, but the start site of transcription is only seven bases from the 5 ' end of the initiator aug codon, making the halcs transcript another example of a " leadless transcript " in the haloarchaea

    它的一29一24位置上是一「毛幻人」 box序列，為典型的嗜鹽古菌啟動子;它的轉錄起始位點距離「 atg 」只有7個核普酸，也是典型的嗜鹽古菌的「卜adless靦script 』 』 。
  15. Pinpointing the worm gene might open the door to drugs that imitate the effects of calorie restriction and could allow people to live longer without following such a severely restrictive diet

    找到蛔蟲這個基因或許能開啟新藥研發之路，以藥物模仿熱量控制的效果，讓人們不用嚴格節食也可以長壽。
  16. The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues, which is part of a alkaline serine protease, with 98. 3 % homogeneity to a reported gene from bacillus pumilus

    序列分析顯示，該片段是一個全長1106bp的orf ，可編碼369個氨基酸殘基的多肽，與短小芽孢桿菌堿性蛋白酶基因編碼區高度同源。
  17. Nucleotide sequencing revealed that the gd gene contained an open reading frame of 1203 bp encoding a 400 aa protein

    基因測序的結果表明gd基因包含一個1203bp的開放性閱讀框（ orf ） ，編碼400個氨基酸組成的多肽。
  18. If the level of the protein is high, the gene is thought to be open. if the level is low, the gene is thought to be close

    因此要研究基因的狀態往往都是考慮這段基因轉錄出來的蛋白質分子的濃度：蛋白質水平高則基因處于開的狀態；蛋白質水平低，則基因處于關的狀態。
  19. The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively

    結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。
  20. In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid

    本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。
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