open-frame 中文意思是什麼
open-frame
解釋
開啟型機座-
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame
重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。 -
The open reading frame of tsarg2 was obtained from human testis cdna library by pcr. using in situ hybridization on tissue section of human testis. we preliminarily studied the expression and function of tsarg2
從小鼠睪丸cdv文庫中分離出該基因完整閱讀框cdna ， ； hu基因的cdna全長為1088hp ，包含6個外顯于，基因組跨越9 -
Based on the contrast with other method, the way " frame + rule " is choused to store the knowledge. directing against the characteristic, put forward positive and negative to the tactics of mixing reasoning. at last adopting the thought of the soft project, the modularized and open system of hydraulic turbine group is realized by delphi 6, which is an object - oriented language
在本文中，研究了水輪機組的狀態監測和故障診斷整個系統，闡明了系統主要內容和總體框架，並著重研究了作為專家系統核心的知識庫及管理系統的構建方法，在比較的基礎上採用了「框架+規則」的組合存儲方法，並且針對特點提出了正反向混合推理的策略，最後採用軟體工程的思想，用delphi6 . 0編程實現了具有模塊化和開放式的水輪機組的故障診斷專家系統。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Chromeplated hexad angles steel pole, auto open frame, glassfiber arms, abs handle, air - jet fabrics cloth, beautiful cartoon pictures dotted, translucent plastic film packing, both for rainy and sunny use
鍍鉻六角鋼桿玻纖骨直桿自動傘架， abs彎柄，防紫外線碰擊布，點綴精美卡通圖案，透膜傘套包裝，晴雨兼用。 -
Chromeplated hexad angles steel pole, auto open frame, glassfiber arms, abs handle, silver paste fabrics cloth, beautiful cartoon pictures dotted, translucent plastic film packing, both for rainy and sunny use
鍍鉻六角鋼桿玻纖骨直桿自動傘架， abs彎柄，防紫外線銀膠布，點綴精美卡通圖案，透膜傘套包裝，晴雨兼用。 -
The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii
並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。 -
Orf, open reading frame
和開放閱讀框架 -
Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector
本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。 -
Badh gene extends 7. 7kb containing 15 exons with the open reading frame of sooaa, 1. 2kb putative promoter region
該基因全長7774hp ，包含15個外顯子， 14個內含子以及約1 -
General construction scheme of rigid - frame open spandrel aarch bridge
空腹式剛架拱橋總體施工方案 -
Three - dimensional photoelastic test of beam - arch joint of open spandrel rigid - frame arch bridge
空腹式剛架拱橋梁拱節點三維光彈性試驗研究 -
General construction scheme of rigid - frame open spandrel arch of youxizhou minjiang river bridge in fuzhou
福州尤溪洲閩江大橋空腹式剛架拱總體施工方案 -
It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species
本論文從水稻和菠菜中克隆了編碼vde酶的基因，並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用，主要獲得了以下結果：首次從兩個水稻亞種（秈稻和粳稻）中克隆了rvde基因（分別命名為irvde和jrvde ）的全長cdna序列，分別長1647bp和1887bp ，兩者開放閱讀框的同源性為98 ，與其它已知vde基因的同源性在60以上。 -
The results of sequencing showed that jl94 isolate complete gene 6 was 1356bp and had a complete open reading frame which encoded 397 amino acides
對克隆的vp6基因進行序列測定，測序結果顯示jl94vp6基因全長1356bp ，含有完整的開放閱讀框架，編碼397個氨基酸。 -
This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。 -
The major portion of the loading on an open frame structure consists of the drag loading.
作用在構架外露的結構物上的載荷，大部分是由拖曳載荷構成。 -
Simultaneously because the world trade organization have carried on negotiations to the telecommunication question, and its documents which have been signatured in fact became the criterion of the telecommunication international trade, this article mainly introduced the content of the agreement on basic telecommunication services and its influence to the telecommunication industry of the countries. after the accession to the wto, the telecommunication service industry of china faced the pressure of the foreign enterprises. this article began with the open frame of the telecommunication industry after the joining of china, and analyzed the chinese telecommunication service industry present situation and the existence disparity
同時由於世界貿易組織對電信問題曾進行了多輪談判，其簽署的相關文件實際上成為電信國際貿易的準則，本文主要介紹了基礎電信協議的大致內容及對各國電信業的影響。加入世界貿易組織之後，中國電信服務業面對來自世界各國電信企業的競爭壓力，本文從加入wto后中國電信業的開放框架入手，分析了中國電信服務業的現狀和存在差距，並論證了中國應積極參與電信頒域國際競爭的觀點，其中既包括中國參與國際市場競爭的可行性，又包括其必要性。 -
Aiming at the problem of how to control an open - frame underwater vehicle precisely, a new method of control based on variable structure neural network was proposed
摘要針對開架式水下機器人運動的精確控制問題，提出一種水下機器人變結構神經網路控制方法。 -
It also leaves more open frame space for storage or jetters
它還留下更開放的存放空間或使用空間。
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