operon gene 中文意思是什麼
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A group of related and closely linked structural genes, together with the operator gene that controls their expression are collectively called an operon.
一組相關的、緊密連鎖的結構基因加上控制其表現的操縱基因,統稱操縱子。 -
Then the engineered genes of bio operon were carried on testing of the functional expression, and biow & biob gene were induced to express
而後對改造后的生物素操縱子基因進行了功能表達檢測及biow與biob基因的誘導表達。 -
Operon is a unit of bacterial gene expression and regulation, including structural genes and cis - acting control elements in dna recognized by regulator gene product ( s )
控制某一代謝途徑的相關基因,緊密連鎖地排列在一起,受同一操縱子控制。 -
However, in the late phase of the growth, the strain hv grew a little faster than e. colihms174. hv ( ptz101 ) was constructed by the plasmid containing the phb operon and the parde gene tansformed into the strain hv
將帶有phb操縱子和質粒穩定分配基因parde的質粒ptz101引入vhb整合菌中,構建成為產phb的vhb整合菌hv ( ptz101 ) 。 -
The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物,首先克隆了鈍頂節旋藻( arthrospiraplatensisfachb341 )藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列( pc - igs )並進行序列測定,然後根據此測序結果設計引物,通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段,其中包括藻藍蛋白亞基基因( cpcb , 519bp ) ,亞基基因( cpca , 489bp ) ,連接蛋白h基因( cpch , 357bp ) ,亞基基因上游啟動子序列( 427bp )以及各基因之間的間隔區( pc - igs , 111bp ; cpch與cpca間隔區, 184bp ) 。 -
Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science
為了證明質粒上的基因能表達出有活性的色氨酸酶,將這個dna片段克隆到pet28c質粒的bamhi和hind位點上,使該片段受t7rna聚合酶的啟動子控制,然後轉化噬菌體de3的溶源菌bl21 ( de3 ) 。
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