overexpressed 中文意思是什麼

The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus

同時，將來源於蘇雲金芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in

從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明，該基因雖然有著細微的種族特異性，但高度保守並獲得了它的截斷型重組蛋白，根據蛋白一級結構和同源比較方法，模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬，預測了它們之間相互作用的特徵。

Clinical valuation and therapeutic target for her - 2 in overexpressed breast cancer

2過度表達的臨床意義及其靶點治療

Intermediate - conductance - ca2 - activated k channels are overexpressed in endometrial cancer and involved in regulating proliferation of endometrial cancer cells

鈣激活性中電導鉀離子通道在子宮內膜癌組織中的表達及其在細胞增殖中的作用

The cdna was subcloned into prokaryotic expression vector pet3d and overexpressed in e. coli bl21 ( de3 )

將該cdna插入原核表達載體pet3d並在大腸桿菌bl21 （ de3 ）中過量表達。

Rynai - w and xynb1 were all cloned into the plasmid vector ppic9 q, and were all overexpressed in pichia pastoris gs l 1 5

將xynb1序列克隆進酵母表達載體ppic9上，在畢赤酵母gs115中得到高效特異性表達。

3 ) western blotting of gas1p when mutant gpi17p overexpressed showed that there was accumulation of immature gas1p. this means that there is a defect in gpi - anchoing

3 ）蛋白質印跡分析表明，在突變gpi17p存在下，有未成熟gas1p的積累，表明存在蛋白質階i化缺陷。

Many scientists have introduced badh gene to plants and got transgenic plants which overexpressed badh, but the accumulations of glybetaine are too low to function effectively

許多研究者已得到了轉badh基因植物，雖然轉基因植物中badh的表達量提高，但是甘氨酸甜菜堿的積累濃度並不高。

The expression products of pel gene was analysis by sds - page and olive oil plate, and the result indicated that pel gene was functionally overexpressed in pichia pastoris and up to 95 % of the secreted protein

表達蛋白pel - gs分泌至培養基中，分子量約28kd ，與pel大小相近，佔分泌蛋白的95 。

The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction

Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。

For prokaryotic expression, the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ). the gene was overexpressed in e. coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction

用pcr方法擴增番紅花八氫番茄紅素脫氫酶（ pds ）編碼區序列，定向克隆到表達載體pet一藝1a （ + ）上，獲得重組質粒pet一21a （ + ） / cspds 。

To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose

為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。