p vector 中文意思是什麼

Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。

Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。

The elements of the load vector, {p}, are the load components tabulated in numerical sequence.

加載向量P的元素是按編號順序列表的加載分量。

Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg

The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。

The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。

In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。

Chen, c. s. 1991. ecological studies on dengue vector in taiwan. abstract of 6th annual meeting of the society for vector ecology. european region, godollo, hungary. p. 9

陳錦生。 1991 。病媒昆蟲分類新技術- - -氣相層析法簡介.行政院環境保護署第四屆病媒防治技術研討會論文摘要集第11頁

Results the number of myocardial and brain and opioid receptors significantly increased, and they were significantly associated with the decreased hemodynamic parameters such as mean arterial pressure ( map ), left intraventricular systolic pressure ( lvsp ), the maximal rate of the change of intraventricular pressure ( dp / dtmax ) and the area of p - dp / dt vector loop ( lo )

結果創傷失血性休克后，大鼠心臟和腦和阿片受體數目明顯升高，親和力無明顯變化，心臟和腦的和阿片受體數目升高與創傷失血性休克后大鼠血流動力學指標下降呈顯著負相關。

Based on the u. s. navy generalized digital environmental model ( gdem ) climatological temperature and salinity data on a 0. 5 x 0. 5 grid, the three - dimensional current system in the kuroshio south of japan and kuroshio extension is determined and analyzed by using the p - vector method

本文選用美國海軍gdem全球氣候月平均溫鹽資料，空間解析度為0 . 5 0 . 5 ，應用p矢量方法對日本以南黑潮和黑潮延續體三維流場進行了診斷計算和分析研究。

The kuroshio meander south of japan and the two lee - wave meanders in the kuroshio extension are well presented by the p - vector method. the path of the kuroshio meander, the position of the meanders in the kuroshio extension, the bifurcation of the kuroshio extension and the width of the current display seasonal variation evidently

日本以南黑潮彎曲路徑呈一「 v 」字型，黑潮延續體西段的大彎曲路徑呈現一「 m 」字型，即「兩峰一谷」的彎曲形態，兩峰分別位於144 e和150 e ，且彎曲程度和彎曲位置都呈現較明顯的季節變化。

The final results obtained in this paper contained that the optimal interpolation scheme highlighted by the covariance that the correlation between different time and the correlation between different place being considered ; that the simplification of kalman filter with the singular - value decomposition ( svd ) and the direct construction of state transition matrix pfeceded with " inverse vector expression " ; and that the analysis of t / p data and its blending with theoretical model

這些成果包括：建立考慮了時間相關的方差矩陣和時空相關的最優插值演算法；對卡爾曼濾波演算法進行了svd簡化以及建立了顯式的狀態轉移矩陣；將t p實時衛星數據進行調和分析並與數值模型進行同化處理。

The method is firstly applied to solve the linear convection - dominated diffusion problems and the optimal l2 - error estimates of the unknown function c and the vector flux p are gained

此方法首先被用於求解線性對流擴散問題，得到了關于未知函數c及伴隨函數p的最優l ~ 2誤差估計。

The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。

The main contributions of the second part of this dissertation are focused on the cryptographic properties of logical functions over finite field, with the help of the properties of trace functions, and that of p - polynomials, as well as the permutation theory over finite field : the new definition of chrestenson linear spectrum is given and the relation between the new chrestenson linear spectrum and the chrestenson cyclic spectrum is presented, followed by the inverse formula of logical function over finite field ; the distribution for linear structures of the logical functions over finite field is discussed and the complete construction of logical functions taking on all vectors as linear structures is suggested, which leads to the conception of the extended affine functions over finite field, whose cryptographic properties is similar to that of the affine functions over field gf ( 2 ) and prime field fp ; the relationship between the degeneration of logical functions and the linear structures, the degeneration of logical functions and the support of chrestenson spectrum, as well as the relation between the nonlinearity and the linear structures are discussed ; using the relation of the logical functions over finite field and the vector logical functions over its prime field, we reveal the relationship between the perfect nonlinear functions over finite field and the vector generalized bent functions over its prime field ; the existence or not of the perfect nonlinear functions with any variables over any finite fields is offered, and some methods are proposed to construct the perfect nonlinear functions by using the balanced p - polynomials over finite field

重新定義了有限域上邏輯函數的chrestenson線性譜，考察了新定義的chrestenson線性譜和原來的chrestenson循環譜的關系，並利用一組對偶基給出了有限域上邏輯函數的反演公式；給出了有限域上隨機變量聯合分佈的分解式，並利用隨機變量聯合分佈的分解式對有限域上邏輯函數的密碼性質進行了研究；給出了有限域上邏輯函數與相應素域上向量邏輯函數的關系，探討了它們之間密碼性質的聯系，如平衡性，相關免疫性，擴散性，線性結構以及非線性度等；討論了有限域上邏輯函數各類線性結構之間的關系，並給出了任意點都是線性結構的邏輯函數的全部構造，由此引出了有限域上的「泛仿射函數」的概念；考察了有限域上邏輯函數的退化性與線性結構的關系、退化性與chrestenson譜支集的關系；給出了有限域邏輯函數非線性度的定義，利用有限域上邏輯函數的非線性度與相應素域上向量邏輯函數非線性度的關系，考察了有限域上邏輯函數的非線性度與線性結構的關系；利用有限域上邏輯函數與相信息工程大學博士學位論文應素域上向量邏輯函數的關系，揭示了有限域上的廣義bent函數與相應素域上的廣義bent函數的關系，以及有限域上的完全非線性函數與相應素域上向量廣義bent函數之間的關系;給出了任意有限域上任意。

The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。

The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。

The recombination expression vector, ppic9 - e2, was linearized by sal i and electroporated into p. pastoris gs115. recombinant p. pastoris gs115, designated gs115 - ppic9 - e2, was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein

重組表達載體ppic9 - e2經sal酶切線性化后，電轉化整合到畢赤酵母gs115基因組上，經pcr鑒定和pcr產物測序分析，陽性轉化子命名為gs115 - ppic9 - e2 。

This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。

In this paper, the p - vector method which is based on - spiral method and thermal wind relation is used and a good result is obtained, which indicates the p - vector method can be used in the large - scale ocean area and invert the current system well

這表明了基於螺旋方法和熱成風關系建立起來的p矢量方法是可行的；該方法在大洋海區有較好的適用性，並能得到較好的診斷結果。