pbv 中文意思是什麼
pbv
解釋
對蝦桿狀病毒-
When the jm109 with pbv / hpk - 5 cultured at a rotating shaker at 30 ? for 3 hours, ph 7. 2, 100ml medium in 500ml flasks, induced at 42 ? for 6 hours, the recombinant protein of rhpk - 5 showed good stability generating
山西醫科大學2002屆碩士學位論文3 .採用「超聲+裂解緩沖液」的方法破碎菌體， 3 %脫氧膽酸鈉有助於溶解少量不溶性的雜蛋自。 -
Four of the six resulting recombinants contained two genes arog and qutb, the other contained three genes arog, qutb and arob. the catalytic activities of both ds and qdhase increased diffierently, but enzyme activities of the recombinant harboring pbv - prpl - qutb - pl - arog were best
六種重組子都實現了目的蛋白的表達，表達產物ds和qdhase酶活性有不同程度的提高，其中pbv - prpl - qutb - pl - arog串聯表達重組子表達產物二種酶活性ds和odhase都比較高，是優化的重組子。 -
Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed
菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。 -
Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs
將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。 -
Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5
Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。 -
4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase
表達載體pbv - a 、 pbv - b和pbv - c經誘導表達，發現所表達的蛋白均為可溶性蛋白，沒有包涵體出現；蛋白經sds ? page分析表明，基因phaa表達的蛋白分子量為42kda ，與?酮硫裂解酶分子量大小一致；基因phab表達的蛋白分子量為26kda ，與乙酰乙酰coa還原酶分子量大小一致；基因phac表達的蛋白分子量為63kda ，與pha合成酶分子量大小一致。
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