peptide mass fingerprinting 中文意思是什麼

peptide mass fingerprinting 解釋
肽質指紋圖譜
  • peptide : n. 【生物化學】肽；縮氨酸。
  • mass : n 彌撒；彌撒的儀式[禱告、音樂]；彌撒曲。 a high [solemn] Mass （有燒香、奏樂等的）大彌撒。 a low ...
  • fingerprinting : 抗體指紋（法）[檢查血清中的特徵性抗體
  1. For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development

    從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
  2. Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion

    差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。
  3. Proteins were identified with peptide mass fingerprinting ( pmf ). firstly, the first molecular anatomic profile of aeromonas hydrophila was established, 40 proteins were identitied from analysis of the pmf of l24 proteins. of the 40 proteins, 17 were from the source of aeromons hydrophila genes / proteins

    實驗首先初步構建了一張嗜水氣單胞菌總蛋白質的分子解剖圖譜，通過對124個蛋白質的肽質量指紋圖譜分析，鑒定了40個蛋白質，其中17個為嗜水氣單胞菌的蛋白。
  4. At first, the gene of eif - 5a was fished from mo - 7e cell line using rt - pcr. then we successfully construct the prokaryotic expression vectors of eif - 5a. after expression and purification, rheif - 5a was identified by western - blot and peptide mass - fingerprinting

    本工作首先從mo7e細胞中提取rna ，通過rt - pcr方法釣取eif - 5a ，在確認序列正確的基礎上，構建eif - 5a的原核表達載體。
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