peptide-elisa 中文意思是什麼

peptide-elisa 解釋
肽-酶聯免疫吸附測定
  1. The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300

    二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。
  2. 3 immunogenicity of m3m4 loop recombinant peptide antigen of nr1, neuroexcitotoxicity protective effects and their mechanisms study of antiserum against m3m4 ( 1 ) to explore the immunogenicity of m3m4 loop recombinant peptide vaccine, m3m4 recombinant peptide was injected subcutaneously to immunize balb / c mice ( h - 2 ), cd4 / cd8 t lymphocytes subsets, the cytokine levels in serum and the liter of specific humoral response were detected with facs, indirect elisa and elisa separately

    ，抗血清還可以結合合成膚nps ，證明該表位具有免疫原性和抗原性。收集抗血清用於功能檢測。 （二） m3m4片段抗血清抗興奮毒性損害作用以臺盼藍染色法和原位末端標記（ tun 』 el ）法觀察抗血清對原代培養海馬神經元興奮毒性壞死和凋亡的保護效應。
  3. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  4. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  5. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  6. These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa

    3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立：為了深入研究細胞因於與配基（受體或抗體）相互作用的關鍵殘基及其空間構象，在上述研究基礎上，我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫，找尋與之結合的tnfa結合肽噬菌體克隆；並以此拓寬了噬菌體肽庫的應用范圍。
  7. Expression of crylac in transgenic tobacco plants was assayed with elisa. the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco

    熒光顯微觀察到gfp在植物細胞間隙高效表達， westernblot結果顯示crylac蛋白也在植物細胞間隙表達。
  8. A synthesised peptide which contain a partial sequence amino acid residues was used in i - elisa, the result is also positive, it means that the amino acid residues 28 - 35 of e2 protein is likely a linear epitope recognized by mcab all

    從而證明核心序列能模擬a11所針對的抗原表位。研究結果提示單抗a11所針對的抗原表位位於e2蛋白的28 ? 35位氨基酸。該區域可能是csfve2一個新的抗原表位。
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