percent detected 中文意思是什麼
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In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
In the study, researchers detected the slimy barrier in 92 percent of children with ear infections who were having tubes placed into their ears
在該項研究中，研究人員在92 %的耳朵受到感染、裝有耳管的兒童耳內發現黏液狀的阻礙物。 -
Glycoprotein bands recovered were detected by gelcodeglycoprotein staining kit, and the result showed that recovery rate reached 50 percent, that the quantity of glycoprotein once purified from sds - gel, was enough to be used in ms, hplc, protein sequence analysis, glycan analysis, and characterization so on
從sds膠上純化的茶樹葉糖肽鑒定結果看，茶樹葉糖蛋白的sds膠上純化回收率達50左右。 -
Theory predicts that gas falling into these monsters will radiate about 10 percent of its intrinsic energy, sufficient to generate a beacon that can be detected on the other side of the universe
理論預測，掉入這些怪物之中的氣體大約會輻射出內在能量的10 % ，足夠產生在宇宙的另一端都偵測得到信號燈。 -
Band of c = o group around 1724 cm " ' was suggested for the characteristic band of pha were observed on the ft - ir spectra of both of the fresh tobacco leaves and their extractions. twenty - five percent of transformed tobacco plants were detected to produce phas
提取物及新鮮轉化植株葉片用傅立葉變換紅外光譜進行定性檢測，在1724cm ~ （ - 1 ）附近有特徵吸收峰確定其為pha ，從而證實有25 . 00的轉基因植株已含有了目的產物。 -
These changes have not been detected at the population level, but e - coli bacteria bugs adapted in lab experiments showed resistance when exposed to as much as 0. 1 percent wt / vol triclosan soap
目前，這種改變（產生耐藥性）還沒有在細菌的群體水平得到證實，但是，當將實驗室常用的大腸桿菌暴露在質量體積比為0 . 1的三氯森香皂環境中時，大腸桿菌產生了耐藥性。
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