permissive strain 中文意思是什麼
permissive strain
解釋
受納菌株- permissive : adj. 1. 許可的，準許的；寬容的。2. 自由的；隨意的。3. 縱容的。4. 【生物化學】受納的，允許復制（遺傳物質等）的。adv. -ly
- strain : vt 1 用力拉，拉緊，抽緊，扯緊。2 使緊張；盡量使用（肌肉等）。3 強迫，強制；濫用，盡量利用。4 拉傷...
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A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
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