phosphotransferase 中文意思是什麼
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In bacterial, many enzymes compete for intracellular pep, especially the phosphotransferase system which is responsible for glucose transport in e. coli. this system uses pep as a phosphate donor and converts it to pyruvate, which is less likely to recycle back to pep
Pep和e4p是dahp合成的限制性底物，在大腸桿菌中， pep又是許多酶的競爭性底物，特別是負責葡萄糖轉運的糖磷酸基轉運系統。 -
Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced
在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。 -
Tpt1 is essential for the vegetative growth of s. cerevisiae and codes for the trna 2 ' - phosphotransferase by using the plasmid shuffling technique, we have constructed a tpt1 - deleted strain and transformed it with trpt1 gene and found that the trpt1 could complement the yeast tpt1
根據trpt1與模式生物酵母tpt1p的同源性，我們構建了tpt1的缺失菌株，利用plasmidshuffle技術，發現trpt1能夠互補酵母tpt1突變的功能，證明trpt1是tpt1的人類同源基因。
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