photolyase 中文意思是什麼

photolyase 解釋
光解酶
  1. Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )

    目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有（ 6 - 4 ）光裂合酶活性的基因，本研究從鹽生杜氏藻dunaliellasalina中克隆到（ 6 - 4 ）光裂合酶的基因，並將該基因在大腸桿菌中得以表達，這是首次在藻類中克隆到（ 6 - 4 ）光裂合酶基因，對光裂合酶的研究具有重要意義。
  2. We report here the cloning of a homolog of this gene from dunaliella salina. this cloned gene produces as protein with photolyase activity when expressd in escherichia coli

    運用blast方法對est序列進行分析，初步預測該est序列為光裂合酶藍光受體蛋白質家族中一個成員的部分cdna 。
  3. 5. after induced with iptg, jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds, cultivate these e. coli with light or without light. the survival rate proved the gene of d. salina ( 6 - 4 ) photolyase has photoreactivation

    5 .工ptg誘導過的轉化菌，經過30秒的紫外光照射后，分別進行光培養或暗培養，計算存活率，從而證明了及sa了z 』 na的（ 6一4 ）光裂合酶在大腸桿菌中可進行光修復活性作用。
  4. It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ), and this study is important for the reseach of 6 - 4 photolyase. the methods and analysis as bellows : 1. this est was analyzed by method of blast, and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor

    利用3 』 race法擴增此cdna序列的3 』端部分，所得片段長度為2575bp ，含一個完整的開放讀框，長1800bp ，可編碼600個氨基酸；利用生物信息學法對此擴增序列進行分析，包括同源性分析、序列的讀框分析、蛋白質的保守性分析以及該蛋白的進化關系分析，預測出此序列編碼了d . salina的（ 6 - 4 ）光裂合酶。
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