physarum 中文意思是什麼
physarum
解釋
絨泡菌屬-
These issues are essential to the understanding of the nature of cell cycle control, and to elucidating the roles of the histone acetylation modification in cell cycle regulation in eukaryotes. in this thesis, we studied the mechanisms of histone acetylation in cell cycle regulation in physarum polycephalum, a naturally synchronized slime mold. the results of this study confirmed that histone acetylation changed the expression of proteins related with the regulation of checkpoint conversion in cell cycle
初步確定了組蛋白乙酰化在多頭絨泡菌細胞周期各轉換點的調控過程中對細胞周期調控相關蛋白表達水平的調節作用；了解了組蛋白乙酰化修飾對細胞周期各轉換點調控的作用途徑；建立了組蛋白乙酰化修飾對多頭絨泡菌細胞周期調控機制的基本模型。 -
Sds - page and western blotting of plasmodia of physarum polycephalum showed that cyclin b was present in the plasmodia of s phase, g2 phase, prophase, and metaphase. the molecular weight of cyclin b in physarum polycephalum was around 62kda. cyclin b was gradually accumulated from s phase to metaphase, and reached the maximal level at metaphase, however disappeared in the plasmodia of anaphase and telophase
Westernblotting分析結果表明， cyclinb存在於s期、 g _ 2期、前期和中期的原質團中，分子量約為62kda ， cyclinb的含量從s期到中期逐漸增加，特別是有絲分裂前期增加明顯，中期原質團cyclinb含量最高，后末期原質團中沒有cyclinb 。 -
4. though tsa treatment and western blotting, we demonstrated that acetylation of certain non - histones may also be associated with the regulation of checkpoints in physarum polycephalum. 5
由此認為，類c fos ，類c jun和類ras蛋白對多頭絨泡菌細胞周期各轉換點調控可能與細胞周期調控相關因於蛋白表達水平的變化有關。 -
To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression
蛋白免疫印跡分析的結果表iv明，當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時，細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平，與未經抗體處理時相比發生了變化（或升高或下降） ；而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少，使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。 -
Based on the data arising from the experiments in this thesis, a hypothesized model, which intends to explain the mechanisms and relationship between histone acetylation and the expression of important cell cycle regulating factors in physarum polycephalum. the main results and conclusions of this thesis are as follows. 1
通過1 」 sa處理、 rt干cr和蛋白兔疫印跡實驗，發現多頭絨泡菌細胞中存在著哺乳動物細胞周期調控相關回于的同源物，包括細胞周期素類cyclinbi蛋白、癌基因產物類c foo和類c j 。 -
By treating the cells in s, g2 phase and prophase with histone deacetylase inhibitor tsa, and through the application of microscopic observation and western - blotting, we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum, affecting the normal crossover of the checkpoints of s / g2, g2 / m and mitosis exit
這些炎白的表達水平具有細胞周期依賴性，隨著細胞周期的進行而發生變化。 tsa處理引起的多頭絨泡菌s期、 gz期和前期細胞內組蛋白h3乙酚化水平的提高，改變了細胞內類cyclinbi蛋白、類。 -
In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1
本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。 -
Mitotic extracts were prepared from the synchronous metaphase plasmodia of physarum polycephalum. the results of western blotting indicated that cyclin b was a component of the mitotic extracts of physarum polycephalum
我們自同步化生長的多頭絨泡菌中獲得了有絲分裂中期提取物， westernblotting分析表明提取物中含有cyclinb 。 -
These factors included cyclin b1 - like protein, p53 - like protein, c - fos - like protein, c - jun - like protein and ras - like protein in physarum polycephalum. 3
Sl和pnranl的mrna以及類hs蛋白的表達水平，從而使細胞周期無法完成s gz 、 gz川以及走出m期各轉換點的過渡。
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