phytase 中文意思是什麼

phytase 解釋
肌醇六磷酸酶；植酸酶
  1. Purification and characterization of phytase from geneticengineering barm

    的純化及性質初步研究
  2. Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied

    植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定，通過初篩和復篩，得到一株產植酸酶較高的黑麴黴（ aspergillusniger ） an00101菌株。
  3. Usin g a silkworm baculovirus expression vector system ( bevs ), a large amount of appa phytase was obtained ( up to 7710 per ml hemolymph )

    感染五齡家蠶后appa植酸酶的表達量達到平均每毫升血淋巴7710u 。
  4. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  5. Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle

    首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。
  6. Specific activity of the rude recombinant phytase is 461575. 8 u / mg

    重組植酸酶粗酶比活力為461575 . 8u / mg 。
  7. Overexpression of selenomonas ruminantium phytase with high specific activity in pichia pastoris

    的高比活植酸酶基因在畢赤酵母中的高效表達
  8. Both the two phytase had a optimal temperature and ph at 60 ? and ph 4. 5 except the baculovirus derived phytase seemed to be more thermostable

    兩個表達系統中表達的重組appa植酸酶的最適溫度與最適ph一致，分別為60與ph4 . 5 。
  9. Monogastric animals, such as pigs and poultry, are not able to utilize phytate phosphorus efficiently, since they have only low levels of phytase activity in their digestive tracts, phytate also acts as an antinutritional agent in monogastric animals by chelating various microelements needed by the animal

    由於單胃動物如豬、雞等的消化道中植酸酶活性很低，導致它們無法有效利用植酸磷。同時，植酸也是一種抗營養因子，因為它能螯合許多動物生長所必須的微量元素。
  10. Effects of dietary phytase supplement on growth performance and body composition of juvenile shrimp penaeus vannamei

    植酸酶對凡納濱對蝦生長性能和體成分的影響
  11. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  12. Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia

    挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。
  13. Phytases are a class of phosphatases that can catalyze the hydrolysis of phytate into myo - inositol and phosphate. it can relieve anti - nutrition of phytate and improve the nutritional value of animal feed from plant. the study on phytase, especially acidic phytase produced by micro - organism, has drawn much attention in home and abroard

    研究表明，飼料中添加植酸酶可使其中磷利用率提高60 ，糞便中磷排出量減少40 ，還可以提高飼料轉化率，改善動物的生產性能，因此對提高養殖業的經濟效益及環境保護均具十分重要意義。
  14. Abstract : this paper mainly reports a. ficuum nrrl3135 phytase " s molecular structure ( phyb and phya ), function of amino acid residues, gene engineerings, biosynthesis and its regulation from molecular biology conclusively, with a view to exploit the development of phytase " s study and application prospects

    文摘:對無花果麴黴的植酸酶分子結構、氨基酸殘基功能及基因工程技術與酶的生物合成、調控等進行論述，並綜述了植酸酶的研究、開發與應用前景
  15. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  16. Expression of gene phya encoding phytase in pichia pastoris and study of properties of phytase

    耐堿性甘露聚糖酶基因的克隆及其在畢赤酵母中的表達
  17. Construction of a double functional recombinant strainof pichia pastoris co - expressing phytase and mannanase and the enzymatic analyses

    植酸酶和甘露聚糖酶雙功能畢赤酵母工程菌的構建和產酶分析
  18. Effects of supplementation of phytase in corn - soybean meal diet on performance of growing pigs

    生長豬玉米豆粕型飼糧中添加植酸酶的效果試驗研究
  19. Over 521 strains were screened for phytase production from 30 kinds of soil samples. out of them, four strains were obtained by the primary screening, then, one strain ( lw03 ), which has higher levels phytase production, was screened by enzyme yielding tests. lw03 strain was primarily identified to be rhizopus

    以lw03為出發菌株經紫外線、 des 、 co ~ （ 60 ）射線和ntg等連續誘變及篩選，得到1株植酸酶高產菌株no13 ，並對根霉no13菌株固態產酶條件、部分酶學性質及其在米糠、菜籽粕植酸體外降解試驗進行了研究。
  20. A non - purified preparation of extracelluar phytase, koji of rhizopus n013, was utilized for predicting inorganic p release from rice bran and rapeseed. adding 300u / kg phytase in rice bran and rapeseed and hydrolysising 4h, the ratio of dephosphorylation of them was 10 % - 20 % and 50 %, respectively, after 16h, were 50 % and 99 %, respectively

    菜籽粕與米糠的體外消化實驗結果表明，在米糠或菜籽粕中添加300u kg植酸酶，於37保溫4h ，米糠植酸磷降解率可達10 20 ，菜籽粕植酸磷降解率可達50 ：保溫16h ，米糠和菜籽粕植酸磷的降解率分別達到55和99 。
分享友人
分享到 Line

分享到臉書