pila 中文意思是什麼
pila
解釋
皮拉-
The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。 -
In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili
本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。 -
In this study, the meaningful results have been achieved, which is the important basic work to research the pili subunit vaccine of avian e. coli, and detect pila gene by nucleric acid probe. moreover, it is significant to research molecular epidemiology, to diagnose, to prevent and treat avian colibacillosis
本研究為雞源致病性大腸桿菌菌毛基因工程疫苗的研究，制備核酸探針檢測pila基因提供了重要材料，對雞大腸桿菌病的分子流行病學研究、診斷和防治研究具有重要意義。
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