plant vector 中文意思是什麼
plant vector
解釋
植物載體-
( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed
（ 2 ）在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ，構建了植物表達載體pbet 。 -
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。 -
Cloning of monomial promoter of actin1 gene and construction of plant expression vector
1的克隆及植物表達載體的構建 -
Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover
選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg -
And then the ced - 9 gene was inserted into plant expression vector pbi121 under the control of camv 35s promoter
在此基礎上再構建重組表達載體pbi - ced9 ，將ced - 9基因置於camv35s啟動子控制之下。 -
2. in order to express chs in plant, we constructed plant expression vector p1301b q c. first added camv 35s promoter and nos terminator to chs by one median clone
（ 2 ）為了能在在植物中表達（ chs ，通過一步的中間克隆，將chs連上camv35s啟動子和nos終止子。 -
By blue - white screening and sequence analysis, we obtained the positive clone. 2. we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation
我們從擬南芥基因組中克隆了dreb1c基因全長，並將其連接到中間載體pgem - t - easy上，進行了測序驗證，結果顯示所克隆的dreb1c序列完全正確。 -
Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。 -
Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed. 3
通過合成六條長鏈引物，經兩次pcr擴增，獲得了238bp的植物防禦素afp基因全長，並構建了植物表達載體peafp 。 -
2. obtaining of mcema ( modified cema ) and plant defensin afp gene mcema ( 141bp ) was amplified with pucspcema plasmid as template and p5, p4 as primers, and plant expression vector pemcema was constructed
Mcema和植物防禦素基因的pcr合成以經測序驗證的spcema為模板，用p _ 5和p _ 4擴增得到了全長141bp的mcema ，並構建了植物表達載體pemcema 。 -
( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
（ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。 -
Construction of plant expression vector with human endostatin gene and transformation of tobacco
利用人內皮抑素基因構建植物表達載體及對煙草的遺傳轉化 -
Construction of vp1 plant expression vector of foot - and - mouth disease virus
1植物表達載體的構建 -
A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene
以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。 -
An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant
為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。 -
A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation
將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。 -
Cloning of egf gene from mouse kidney and construction of its plant expression vector
基因的克隆及其植物表達載體的構建 -
Construction and identification of vp1 plant high efficiency expression vector of foot - mouth disease virus
1高效植物表達載體構建及鑒定 -
Cloning of a carrot gene encoding antifreeze protein and construction of its plant expression vector
胡蘿卜抗凍蛋白基因克隆及植物表達載體構建 -
Plant expression vector pespcema was constructed after subcloning in puc121 and other vectors
經puc121等中間載體的亞克隆，構建了pespcema植物表達載體。
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