plasmid analysis 中文意思是什麼

plasmid analysis 解釋
質粒體分析
  • plasmid : 質粒；原核質體
  • analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication

    從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  5. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  6. Complete sequence analysis of the two plasmids reveal that the small plasmid plp2000 with 2061 nucleotide encodes a putative 37 kda replicase. and in the downstream region of the replicase gene, it existed multi - tandem repeat of 17 bps

    Southernblot顯示plp2000和plp9000兩種質粒屬于滾環復制類型質粒，和滾環復制相關的鉆序列可能形成的二級結構分別為sso和ssou類型。
  7. The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod

    結果表明同源性分別達到98和97 ，並且在ha1切割位點有多個堿性氨基酸的插入序列，證明其為強毒株。
  8. Methods : plasmid profile analysis was used

    方法：堿裂解法。
  9. Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5

    Sds - page分析表明，重組質粒pet - 32a （ + ） - igf -在iptg誘導下表達分子量約30kda的融合蛋白，但其表達量不高，約為菌體總蛋白的10左右；重組質粒pet - 30a （ + ） - igf -在iptg誘導下表達分子量約14kda的重組蛋白，融合蛋白表達量約占菌體蛋白總量的35 。
  10. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  11. Cloning and sequence analysis of ebv - lmp1 cdna and the constuction of prokaryotic expression plasmid

    的克隆測序與原核表達質粒構建
  12. Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program

    利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。
  13. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  14. The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively

    結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。
  15. Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing

    通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。
  16. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  17. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  18. Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g

    為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。
  19. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  20. Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121, indicating that t - dna had been integrated into the genome of sweet cherry

    L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。
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