plasmid dna 中文意思是什麼

plasmid dna 解釋
質粒
  • plasmid : 質粒；原核質體
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. Protective immunity to schistosoma japonicum elicited by co - immunization of cathepsin b dna vaccine with eukaryotic plasmid encoding il

    4真核表達質粒聯合免疫誘導小鼠保護性的研究
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Sedegah m, hedstrom rc, hobart p, et al. protection against malaria by immunization with plasmid dna encoding circumsporozoite protein [ j ]. proc natl acad sci usa, 1994, 91 ( 21 ) : 9866

    劉彥文，余新炳，徐勁等.惡性瘧原蟲海南株環子孢子蛋白基因的克隆與表達[ j ] .中國人獸共患病雜志， 2000 ， 16 （ 1 ） : 8
  5. An improved alkaline lysis adaptive to extracting plasmid dna of bacillus

    提取的改良堿裂解法
  6. In two of these isolates with typical class 1 integron, it was showed that the integrons were both harbored in plasmids after intll southenbloting both on their genome dna and plasmid dna

    1株福氏志賀菌y變種攜帶的基因盒類型與其中一株宋內志賀菌相同，為護漢17和aad減萬。
  7. It was showed under the laser scanning confocal microscopy that the transfected plasmid dna transcripted by rna polymerase and its transcripts were both localized in the interior and periphery of nucleoli

    由此我們認為rna聚合酶的轉錄就是發生在這一區域里，而並非是人們傳統認識中的核質中。
  8. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  9. The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only

    對於野生菌株的質粒提取，需要選擇合適的提取方法，我們首先採用了細菌細胞質粒提取常用的堿裂解法，但是未取得理想的效果，只得到了許多破碎的質粒dna片段。
  10. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  11. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  12. A research for methods of extracting plasmid dna

    提取與純化方法的研究
  13. In conclusion, comparing with low let radiation, the higher let heavy - ion radiation to purified plasmid dna has more efficient biological effectiveness

    綜上所述，高let重離子輻射射線與低let輻射射線相比，呈現出較高的生物學效應。
  14. Curcumin - induced oxidative damage of plasmid dna

    氧化損傷的體外實驗
  15. Pad. hcv - c, pad. hcv - cel and pad. hcv - ce ! e2 were packed and screened by virus plaques technique, identified to be good infectivity and expressivity of inserted hcv structural proteins or fusional structural. in contrast to plasmid dna vector, adenovirus vectors appeared to be better immunizational effects and with the hope of being developed to be vaccine against hcv

    Hcv ceiez ，經鑒定具有較好的感染性，能較好的表達插入的hcv結構蛋白或融合結構蛋臼，與質粒dna載體相比，腺病毒載體具有更好的免疫效果，可望發展成為抗hcv感染的腺病毒載體疫苗。
  16. The extraction results show that the clear and integral plasmid dna bands were got using these two methods

    結果表明，應用這兩種方法可以得到清晰完整的質粒帶。
  17. Switch on the vacuum to draw the solution through the column ( the plasmid dna binds to the column material )

    開啟真空裝置使液體通過管柱（質體dna會與管柱物質結合） 。
  18. And p4. 8 comparison were performed between the 3 t - cell vaccine and plasmid dna vaccine expressing hcv structural gene on therapeutic effects in hcv transplanting tumor murine model. the result showed that the former was obviously superior the latter, which indi

    將3種丁細胞疫苗與表達v結構基因的質粒a疫苗在v移他瘤小鼠捎型內進行治療對比，發現djj者明顯憂於後者，提示， t細胞疫祈在ncv感染的防治研究中可能具有較奸的發展汕景。
  19. A mutant probably harboring more plasmids can grow better on nitrobenzene selective medium than other strains. the plasmid harbored in this mutant is also a few kb smaller than others. so a probability is supposed that a certain plasmid dna fragment deletion made the number of plasmid copy change, which affected the mutant growth on the selective medium

    一株質粒含量較高的突變株在硝基苯選擇培養基中的生長情況要好於其它菌株，其所含的質粒也略小一些，因此我們推測，某個質粒片段的缺失造成了質粒拷貝數的變化，從而影響了它的生長特性。
  20. The plasmid dna was cut and dephosphorylated. the linearized plasmid dna was ligated to the pseudomonas sp. dna fragments and resulting recombinant plasmids were used to transform escherichia coli xll - blue

    提取細菌總dna ，用限制性內切酶部分酶切后，回收2一6kbdna片段，與克隆載體puc18連接，轉化大腸桿菌，構建了該菌株的基因組文庫。
分享友人
分享到 Line

分享到臉書