polyadenylation 中文意思是什麼

polyadenylation 解釋
多聚腺苷酸化
  1. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  2. A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon

    在其起始密碼子上游- 65nt發現桿狀病毒晚期啟動子轉錄起始信號ataag ，在其終止密碼子下游- 14nt發現polya加尾信號aataaa 。
  3. But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products

    細菌mrna多聚腺苷酸化的位點多種多樣，包括初級轉錄產物的3 』末端， 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內，其腺苷酸化相對無特異性、無選擇性。
  4. It has been known that polyadenylation of 3 " end of mrna in prokaryotes is different from that of eukaryotes. the poly ( a ) tracts in bacteria are generally short, ranging from 15 - 60 adenylates residues and associated with only 2 - 60 % of the molecule of a given mrna species. mrna polyadenylation of eukaryotes always occurs in the untranslated region, 10 - 30 nucleotides downstream of the hexanucleotide sequence aauaaa

    現在普遍認為細菌許多mrna3 』端存在多聚腺苷酸尾，但其長度通常較短，大約在15 - 60腺苷酸殘基范圍，且對某一mrna而言，僅有2 - 60的分子被多聚腺苷酸化。
  5. Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20. 7kd, 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail. 3

    棉花arf基因的序列分析核苷酸序列分析表明， gharf的全長為828bp ，其中含有一個從131bp到676bp間的546bp構成的一個完整開放閱讀框，它編碼181個氨基酸、分子量為20 . 7kd的蛋白質。
  6. 2. a new framework, named information entropy model of sliding window ( iemsw ), was proposed to effectively analyze the structural information contained in the 3 ' - utr sequences around the polyadenylation site. 3

    為有效地分析水稻3 - utr序列剪切位點上下游序列中的信息結構，提出了一個新的分析框架，即dna序列的滑動窗口信息熵模型。
  7. And then a 378bp acci fragment in pbtk2. 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni, a eukaryotic expression plasmid

    在此基礎上，進一步用tthlll酶切后，補平插入帶有sv40啟動子的大腸桿菌?半乳糖苷酶報一告基因（ lacz ） ，獲得通用轉移載體pltk - uni 。
  8. Our research demonstrates that rd - pcr is an effective technique for constructing cdna fragments libraries in bacteria, which can greatly decrease the redundancy of cdna libraries, leading to reduction of time and labor in our further endeavor to prepare cdna microarrays. we assessed changes in rna levels after exposure to heat shock using the microarray and found the polyadenylation level of mrna significantly decreased after exposure to heat shock

    實驗研究發現限制性顯示pcr技術可以很好地用於細菌的poly （ a ）化mrna的限制性cdna文庫的構建，巧妙地解決了由於細菌mrnapoly （ a ）化位點的高度多態性，用常規方法構建原核cdna文庫所遇到的文庫大量重復冗餘的難題。
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