pre-incubated 中文意思是什麼
pre-incubated
解釋
預培養的,預孵化的-
In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。 -
Based on epidermal strip bioassay, microinjection, patch - clamp and laser scanning confocal microscopy in the experiments, we provided the first evidence that map kinases, including mek1 / 2 or p38 / hog1, plays an important role i n aba - or sa - induced h2o2 signal initial, amplification and specific targeting in response to stimuli in guard cells. aba - or h2o2 - induced vicia faba stomatal closure. was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059, aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf )
在對照實驗中， aba誘導熒光迅速增高；單獨的pd98059 、 pd98059和aba共同處理氣孔時，保衛細胞內h _ 2o _ 2探針熒光強度沒有增高；將pd98059注射進入其中的一個保衛細胞，再以aba處理，使得兩個有同樣熒光基礎的保衛細胞熒光強度對比強烈；將pd98059顯微注射進入已被aba誘導dcf （ dichlorofluorescin ）熒光強度升高的氣孔保衛細胞，熒光強度下降，而沒有被注射一邊的保衛細胞中的dcf熒光強度不變。
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