prokaryotic vector 中文意思是什麼

prokaryotic vector 解釋
原核載體
  • prokaryotic : 初核質
  • vector : n 1 【數學】向量，矢量，動徑。2 【航空】飛機航線；航向指示。3 【天文學】幅，矢徑。4 【生物學】帶...
  1. Construction of the prokaryotic expression vector of mtb lhp gene and its expression

    基因原核表達載體的構建和表達
  2. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  3. Cloning and construction of prokaryotic expression vector of vp1 gene of foot - and - mouth disease virus serotype o

    1基因的克隆及原核表達載體構建
  4. Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus

    但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達，而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。
  5. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  6. In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon

    序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。
  7. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  8. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  9. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  10. In this study, by combining rt - pcr and race, a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations

    本論文研究工作採用rt - pcr與race相結合的方法，首次克隆了草原兔尾鼠zp3全長cdna並進行了序列分析，構建了真核表達質粒，為草原兔尾鼠免疫不育的研究奠定了基礎。
  11. While there are some difference in amino acid with other group ( except tw 95 ), the amino acid in 65 - 75 of hn of ndv group are conserved, while he in 81 is conserved. based on epitope prediction, five pairs primers are designed with enzyme sites. five segments of f1, f2, f3, f4 and hn1 coding epitope was amplified and cloned to prokaryotic expression vector, respectively

    在表位預測的基礎上，將臨近的表位合併，設計引物並引入酶切位點，以fa 、 fb 、 hna為模板擴增含抗原表位的多肽片段f1 、 f2 、 f3 、 f4 、 hn1 ，克隆到原核表達載體ppro ~ （ ex ） ht上，成功地構建了五個表達載體。
  12. The cdna was subcloned into prokaryotic expression vector pet3d and overexpressed in e. coli bl21 ( de3 )

    將該cdna插入原核表達載體pet3d並在大腸桿菌bl21 （ de3 ）中過量表達。
  13. For prokaryotic expression, the coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into a prokaryotic expression vector pet - 21a ( + ). the gene was overexpressed in e. coli bl21 ( de3 ) and gave rise to a 63 kd mature protein in response to the iptg induction

    用pcr方法擴增番紅花八氫番茄紅素脫氫酶（ pds ）編碼區序列，定向克隆到表達載體pet一藝1a （ + ）上，獲得重組質粒pet一21a （ + ） / cspds 。
  14. To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose

    為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。
  15. Modified crylle and crylac gene were cloned into prokaryotic expression vector pet28b, to construct plasmid petie and petac

    將這兩個基因構建到原核表達載體pet28b中，構建成原核表達載體petac和petie 。
  16. So, we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein

    為此將emt l氨基末端基因克隆入融合表達載體prsetb中，轉化大腸桿菌bl 21 ，經iptg誘導，表達his6 emt l融合蛋白，表達產物分子量約為24ku 。
  17. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
  18. A prokaryotic expression vector was succsessfully constructed and transformed in to e. coli expression strain bl21 ( de3 ). with the iptg induction, a 50kd protein of - 6 fatty acid desaturase is expressed

    原核表達載體petfad2轉化到表達菌株bl21 （ de3 ）后，經iptg誘導得到了目標蛋白。
  19. The purified bovine follistatin cdna was inserted into pet - 30a vector to construct the prokaryotic expression vector pet - 30a - fs2

    經正反測序表明其與genbank中發表的牛follistatincdna序列完全相同。
  20. 2. to construct the prokaryotic expression vector and the control vector. after sequenced, the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr

    將自puc18 h d - 3回收的目的片段連同表達運載蛋白dhfr的基因片斷一起連接于原核高效表達載體pqe - 80l ，經酶切鑒定，得到重組原核表達載體pqe - 80l dhfr h d - 3 。
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