promoter site 中文意思是什麼

promoter site 解釋
啟動部位
  • promoter : n. 1. 增進者，助長者；振興者，獎勵者；後援人；（通常指惡意的）煽動者。2. （企業等）發起人；推銷者。3. 【化學】促進劑，助催化劑。4. （宗教裁判的）起訴人。
  • site : n 1 地點；位置；地基。2 場所，現場。3 遺址。4 【計算機】網站，站點〈電腦網路用戶的網站地址〉；萬...
  1. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  2. Promotor, in broad sense, consists of transcription start site, binding site of rna polymerase ( promoter in narrow sense ) and upstream regulation sequence

    廣義的啟動子（ promotor ）包含有轉錄起始部位、 rna聚合酶與dna結合部位（也就是狹義的啟動子）以及上游調控序列。
  3. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  4. The entire halcs gene is located on a megaplasmid and contains a 849 - bp open reading frame that encodes a polypeptide of 283 amino acids. the promoter is typically haloarchaeal, but the start site of transcription is only seven bases from the 5 ' end of the initiator aug codon, making the halcs transcript another example of a " leadless transcript " in the haloarchaea

    它的一29一24位置上是一「毛幻人」 box序列，為典型的嗜鹽古菌啟動子;它的轉錄起始位點距離「 atg 」只有7個核普酸，也是典型的嗜鹽古菌的「卜adless靦script 』 』 。
  5. By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter, two site - mutation promoters, ipms ( 603 bp ) and ipm1 ( 900 bp ) were created

    通過pcr方法對克隆的兩個啟動子進行定點突變，使轉錄起始位點上游- 137bp處a突變為c ，得到兩個突變啟動子（ ipml 、 ipms ） 。
  6. 2. specific mutation from a to c at - 137 bp site upstream from transcription start point had no effect on the inducibility of the promoter in response to sa and bth. 3

    在轉錄起始點上游137bp定點將a突變為c后，對化學啟動子的化學誘導應答性沒有明顯的影響，但對誘導效應的向上運輸存在一定程度的阻抑作用。
  7. Activity of each construct was determined. the basal promoter was located at about 60bp up stream of the transcription initiation site. it contains a tata box at - 33bp which is required for the transcription initiation

    基因的基本啟動子元件位於轉錄起始位點上游約60bp ，其中含有一個位於- 33bp處的tatabox ，它對于轉錄起始起著重要作用。
  8. 900 bp promoter - directed gus expression was highly induced by sa and bth, while the 603 bp promoter, whether mutated or not, did not respond to sa and bth induction, which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site

    全長900bp啟動子能夠應答sa和bth的誘導，而603bp長的啟動子無論突變與否對sa和bth均無應答，證明sa和bth的應答區域在克隆啟動子的轉錄起始位點上游575 872bp之間。
  9. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  10. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  11. The pa7 fragment was sequenced and several motifs similar to prokaryotic promoter elements such as - lobox, - 35box and up box were found. the sd sequence which was bound by ribosome and atg site were also found. the pat fragment was blast in genebank, but there is no its " homological sequence

    對pa7片段的序列分析發現其距5 』端931bp ? 1091bp處具有原核生物典型基因啟動子的保守結構- 10區和- 35區，以及翻譯必需的sd序列和翻譯起始位點等。
  12. It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation. a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter

    （ 465 1079氨基酸段）對sv40啟動子明顯的抑制活性，因為它只包含dna結合域和核結合位點，說明發揮轉錄抑制功能的關鍵是ie180的dna結合域。
  13. To correlate microarray data with the promoter site consensus sequence for a specific transcription factor

    找出微陣列資料與特定轉錄因子的促進子共識序列之間的相互關聯性。
  14. Cat ( chloramphenical acetyl transferase ) elisa assays to identified the function of constructed plasmid. the result indicates mutant p1p2, p1p3p2 and p6p2 repressed to cmv promoter. same as ie promoter two special sequences " atcgt " located at two side of the transcription initial site of promoter

    共轉染hplp細胞，結果表明所有的缺失突變體對cmv啟動子都表現抑制活性，我們發現在ie180和p啟動子的轉錄起始位置兩側同時具有兩個特徵序列（ 5 』 atffit 3 』 ） 。
  15. In abroad, the study of integration site used for transgenic detection had just begun. in this study, according to the collection of the global commercialized transgenic crops, select seven exogenous genes which basically cover the total commercialized crops, namely camv35s and fmv promoter, nos terminater, mark gene nptii, and aim genes pat, epsps and cryia ( b ). use endogenous 18srrna gene as collate, design a large pairs of specific primers, screen the optimum primers groups, optimized the test condition and parameters, establishing the qualitative pcr detection system

    本研究根據收集的國內外已商品化的轉基因作物品種，選擇了能基本覆蓋商品化轉基因品種的7個外源基因，即： camv35s 、 fmv啟動子、 nos終止子、 npt標記基因和目的基因pat 、 epsps 、 cryia （ b ）作為篩選目標，以植物18srrna基因作為內源參照基因，設計了多對特異性引物，並篩選出最佳組合，優化了檢測條件和參數，建立了pcr定性檢測方法體系。
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