protein fingerprint 中文意思是什麼

protein fingerprint 解釋
蛋白指文圖
  1. 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared

    二是通過原位酶解,抽提蛋白質片段進行maldi - tof質譜分析,利用肽質量指紋圖數據在數據庫中進行檢索,在雙向電泳凝膠上取了57個點進行分析,有34個蛋白質點在數據庫得到歸屬鑒定。
  2. Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis, biological mass spectrometry and bioinformatics. in 2 - de, we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples. in the identification of 10 proteins of serum and 16 proteins of skin, we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms )

    研究方法:採用雙向電泳技術獲取泥鰍創傷前後血清和皮膚的小分子蛋白圖譜,利用基質輔助激光解析飛行時間檢測質譜分析( matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry , maldi - tof - ms )和電噴霧離子化串連質譜分析( electrosprayionizationms / ms , esi - ms hs )分別獲得差異蛋白點的肽指紋圖譜( peptidemassoffingerprint , pmf )和部分序列信息,通過網際網路上的expasy服務器中和ncbi的相關軟體將這些信息和swissprot數據庫進行匹配鑒定蛋白質種類。
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