protein gene product 中文意思是什麼

protein gene product 解釋

  • protein : n. 【化學】朊，蛋白（質）。
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • product : n. 1. 產物，產品；製品；產量；出產。2. 結果，成果。3. 創作，作品。4. 【化學】生成物；【數學】積，乘積。
  1. It still remains a question whether the rearrangements of igh come from h / rs cell or the background lymphocytes. in this study, we have detected the igh clonal correlation between the h / rs cells and the background cells, from a new aspect to study the clonality of h / rs cell and its relation with the background cells. the expression of b - cell - specific activator protein ( bsap ) was detected in hl. igh gene rearrangements were analysed by the methods including gene analysis in neoplasms tissue and micropicked cells from paraffin - embedded sections, sequencing to test the pcr product, and in situ pcr

    本研究將在以往研究的基礎上，在國內率先把b細胞核反式作用因子? b細胞特異性激活蛋白（ b - cell - specificactivatorprotein ， bsap ）應用於hl的研究，檢測hl的bsap表達，並採用石蠟刮片組織和微切割單細胞的基因分析、測序分析和間接原位pcr等方法，同步觀察分析h rs和背景淋巴細胞的igh基因克隆相關性，從又一個新視角探究chl的腫瘤性h rs細胞克隆性及與背景淋巴細胞的關系。
  2. Deficiency of apoe may promote to produce and develop atherosclerotic lessions. the apoe gene - targeted mice will result in marked regression of both early and advanced atherosclerotic lesions by injected apoe recombinant protein, or by transfected adviral vector with apoe cdna to express human apoe transgene in liver, or by transplantation of bone marrow with normal rat apoe gene. this demonstrates that apoe gene and its expressing product can inhibit progression of atherogenesis. apoe3 has a more effective prevention from as than apoe2 and apoe4

    Apoe的缺失可促進動脈粥樣硬化的發生發展，給apoe基因敲除鼠反復注射apoe重組蛋白、在肝組織中用腺病毒載體表達apoe蛋白、移植帶有正常apoe基因的小鼠骨髓，都能使apoe基因敲除鼠的動脈粥樣硬化得到回復，表明apoe基因及其表達產物對動脈粥樣硬化的發生具有抑制作用， apoe _ 3對動脈硬化的阻抑作用要比apoe _ 2和apoe _ 4都明顯。
  3. 97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found

    將含有重組質粒phn的菌株dh5在37條件下培養，以濃度為0 . 6mmol / liptg誘導，重組質粒n基因phn融合蛋白獲得了表達：經sds - page ， western - blot試驗，確定其表達的融合蛋白產物大小為預期的47ku 。
  4. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  5. Howeer, protein gene product 9. 5 - immunoreactie fibers were obsered in scar tissues on the surface of the puncture site

    但是，在穿刺處表面，我們找到了9 . 5蛋白基因產物的免疫反應纖維。
  6. In all the discs, including nonpunctured control discs, protein gene product 9. 5 - immunoreactie fibers were only occasionally obsered in the outermost part of the anulus fibrosus

    在包括未穿刺的對造組在內的所有椎間盤中， 9 . 5蛋白基因產物的免疫反應纖維只是偶爾在環狀纖維的最外邊被觀察到。
  7. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  8. The amount of the goal protein was evaluated by densitometric scanning. it indicated that the product of the vp2 gene was 14 % of total bacterial protein of dh5a

    表達產物經ni - nta金屬鏊合層析柱純化后，得到了純度較高的6his - vp2融合蛋白。
  9. Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker

    綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。
  10. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  11. The amount of the goal protein was evaluated by densitometric scanning. it indicated that the 45ku product of the vp6 gene was 26. 5 % of total bacterial protein of bl21

    表達產物經ni - nta金屬螯合層析柱純化后，得到純化的his - taggedvp6融合蛋白。
  12. A 12 bp sequence of the 5 " end from the polyhedrin protein gene of bmnpv was ligated to the 5 ' end of hbsag ( pres2 + s ) protein coding sequence by pcr. the fusion product coding for hbv surface antigen medium sized ( hbmp ) with 4 - additional aa of bmnpv polyhedrin protein was obtained

    本研究通過pcr突變的方法，在hbsag （ pres2 + s ）前s2序列的5 』端融合了bmnpv多角體蛋白基因5 』端的12個堿基，獲得了融合乙肝表面抗原中蛋白基因（ hbmp ） 。
  13. The absence of the fmr1 gene product, fragile x mental retardation protein ( fmrp ), is believed to be responsible for the typical physical and mental characteristics of the fragile x syndrome. alleles with between 43 and 200 cgg repeats are called permutation. they are generally unmethylated with normal transcript and protein level, but are extremely unstable during transmission to next generation

    帶有前突變（ n = 43 200 ）的個體其fmr1基因通常不會甲基化，可以正常轉錄和翻譯產生fmrp ，故沒有臨床表型出現，但是，在向下一代傳遞的過程中卻是非常不穩定的，會從前突變擴增成為全突變。
  14. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  15. These results suggested that the 65kda protein was a degraded product, bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella. 3. expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent, a new simple transformation method of pf was established

    3 .用上述重組技術獲得了含有刀口基因的lz20和含有屍2拼即「的lz20a克隆株，通過基因的誘導表達，發現p 」和pz瀏民有不同的作用機制，證明了p20具有保護cyt人溶細胞的作用，這種作用並不依賴大腸桿菌受休的不同而改變
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