receptor assay 中文意思是什麼
receptor assay
解釋
受體分析-
Development of a functional reporter gene assay for the identification of glucagon - like peptide - 1 receptor agonist
1受體激動劑功能性報告基因分析系統的構建 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization
本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。 -
However, the lack of a simple, sensitive, and reliable method for mersuring mt has been an obstacle to the elucidation of its physiological and pathological role [ 3 - 5 ]. after initial attempts with bioassay, gas chromatography - mass spectrometry for quantifying mt in body fluids was tried, followed by hplc. the major limitations to these techniques were expense, low throughput, extensive sample work - up before assay [ 4, 5 ]. in order to further study the funcation of pineal gland, also in order to deeper understand drug - receptor interactions, therefore better to build mt receptor models and to design new seletive ligands. which applying solid foundation for mt and its analoges ' s therapeutical application. we produce and characterizate monoclonal antibodies to melatonin
Kx碩士掌位論文wmaster 』 stiffisis在最初嘗試用生物檢測法、氣相色譜一質譜來檢測mt在體液中的量，后來通過高效液相色譜6 8 ，這些技術主要的限制是過于昂貴檢測樣品少、檢測前樣品處理復雜3 ， 4 。為了更進一步地研究松果體的功能，也為更深入了解藥物受體的內在反應，為建立更完善的mt受體模型和設計新的可選性配體，為mt和其類似物治療應用提供堅實的基礎，我們制備和鑒定了抗mt的單克隆抗體。 -
In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。 -
Methods receptor binding assay ( rba ) was adopted to measure the distribution of pgr and er in liver cancer and tissue in its vicinity in 63 patients with primary liver cancer ( plc ) undergone laparotomy and in non - tumor liver tissue in 7 cases with gastric carcinoma
方法採用放射性配基結合分析法（ rba ）測定了63例行手術切除的肝癌組織、癌周組織及7例非肝癌肝組織的雌、孕激素受體的含量並結合臨床資料進行統計分析。 -
The authors develop a protein - fragment complementation assay that specifically evaluates androgen receptor ligands in the context of signal activation
作者開發一種蛋白質片段互補分析，具體評價激素受體配體的背景信號激活 -
Gorgoulis v, aninos d, mikou p, et al. quantitative assay of epidermal growth receptor in squamous cell lung carcinomas, anticancer res, 1992, 12 : 1183
鄭唯強，倪燦榮，龔志錦，等.乳腺癌上皮生長因子受體的臨床病理學觀察及預后意義.癌癥， 1995 ， 14 （ 1 ） : 19
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