recombinant bacteria 中文意思是什麼

recombinant bacteria 解釋
重組細菌
  • recombinant : n. 【遺】重組器官，復合器官。
  • bacteria : n. pl. （sing. Bacterium ）1. 細菌。2. 〈美俚〉拳擊迷。
  1. Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique.

    近年曾經應用重組DNA技術使細菌產生干擾素獲得了一定成效。
  2. Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug

    許多研究表明重組內皮抑素特異性抑制內皮細胞增殖，而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在，將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白，幾乎完全抑制鼠lewis肺癌等多種腫瘤生長，並無耐藥性產生，即使中斷治療腫瘤也不再復發。
  3. Then bacteria were collected by centrifugation and split by ultrasonic method. the purified products were analyzed by tris - tricine sds - page. the immuno - activity of the recombinant proteins were analyzed by western blot

    將離心收集到的菌體超聲破壁，離心分離，收集上清液，進行離子交換層析和分子篩分離純化。
  4. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  5. Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5

    Sds - page分析表明，重組質粒pet - 32a （ + ） - igf -在iptg誘導下表達分子量約30kda的融合蛋白，但其表達量不高，約為菌體總蛋白的10左右；重組質粒pet - 30a （ + ） - igf -在iptg誘導下表達分子量約14kda的重組蛋白，融合蛋白表達量約占菌體蛋白總量的35 。
  6. The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5

    將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。
  7. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  8. The mrna and protein expression were assayed by rt - pcr and sds - page. the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )

    以iptg誘導融合蛋白表達，經rt - pcr檢測發現740bp的特異性il - 6條帶；通過sds - page分析，在49kd處出現gst ? pil6融合蛋白條帶，其表達量占總細菌蛋白量的30 ，證明豬il - 6基因得到了正確轉錄和表達。
  9. The recent development of fuel ethanol industry was introduced in this paper and the research progress of various ethanologenic recombinant enteric bacteria was reviewed

    本文介紹了燃料酒精工業發展的最新動態，並對多種產乙醇重組腸道細菌的研究進展作了綜述。
  10. These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria. the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit, and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128, 000

    從制備性聚丙烯酰胺凝膠中回收融合蛋白，用mtt法測定重組蛋白刺激小鼠脾淋巴母細胞增殖反應活性，並以重組蛋白免疫家兔制備抗豬il - 6滴度為128 ， 000的高免兔血清。
  11. The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23. 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )

    Rt - pcr分析確證重組質粒在大腸桿菌中能正確轉錄； sds - page分析證明誘導重組質粒表達了融合蛋白，分子量約為50kd ，與預期的26kd的gst帶和23 . 6kd的草魚生長激素基因編碼蛋白質構成的融合蛋白大小一致。
  12. Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique

    近年曾經應用重組dna技術使細菌產生干擾素獲得了一定成效。
  13. Section hi : purify & refold recombinant hpk - 5 protein was efficiently expressed in e. coli jm109 as inclusion bodies. after bacteria were smashed by ultrasound, te buffer, 1 % ttiton x - 100 and 2 m urea was used to efficiently extract inclusion bodies

    用含sm尿素溶液洗滌包涵體， 8000r / min轉速下分離包涵體，能最大限度去除雜蛋白，同時不會降低目的蛋白的損失。
  14. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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