recombinants 中文意思是什麼

Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e. coli strain dh5 - alpha to generate cdna library that size is 4. 9 l06 recombinants

將cdna與載體連接，並導入dh5感受態細胞中，構建成cdna文庫。

But as to muts recombinants, the periods of fermentation is too long and the efficiency of using methanol is low

然而， mut ~ s菌株由於發酵周期長，甲醇率利用低，並不適用於實際的大規模生產。

To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions

在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。

Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。

Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。

Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

The yield reached 10 - 12mg / l. dr. chen ding hu in plant virus group of institute of plant protection of the caas ( chinese academy of agriculture sciences ) selected pap muts recombinants and wanted to produce pap through fermentation to prevention and cure plant virus disease

中國農科院植保所植物病毒實驗室陳定虎博士成功的篩選到p . pastorispap利用甲醇緩慢型重組子（ mut ~ s ） ，希望能利用微生物發酵的方法來大量生產pap ，將其應用於植物病毒病的防治。

A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate

以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。

Construction and immunogenicity of multi - epitopes recombinants

流感復合多表位疫苗的設計及小鼠免疫研究

The library size is 1. 2x 106 recombinants. the average length of inserted fragments in the library was longer than 450bp, which was testified by both pcr and sequencing analysis

藍白斑檢驗結果表明cdna文庫滴度為1 . 2 10 ~ 6 ，重組效率為95 . 9 ， pcr檢測及序列分析結果驗證插入片段平均長度大於450bp 。

The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations

經甲醇誘導表達后， sds - page和westernblot結果證明了p . pastoris培養上清液中含有正確糖基化的e2蛋白，表達量約250mg / l 。

Four of the six resulting recombinants contained two genes arog and qutb, the other contained three genes arog, qutb and arob. the catalytic activities of both ds and qdhase increased diffierently, but enzyme activities of the recombinant harboring pbv - prpl - qutb - pl - arog were best

六種重組子都實現了目的蛋白的表達，表達產物ds和qdhase酶活性有不同程度的提高，其中pbv - prpl - qutb - pl - arog串聯表達重組子表達產物二種酶活性ds和odhase都比較高，是優化的重組子。

The pcr products were purified and linked with pmd 18 - t vector respectively. the positive recombinants were selected and digested with double restriction enzymes respectively. goat fsh - a and p subunit genes which had sticky ends were purified and linked respectively with pf which also had the corresbonding sticky ends and were purified

將所得的山羊fsh - 、亞基基因分別用加相應限制性內切酶酶切位點的引物大量擴增，回收后與pmd18 - t連接，篩選出陽性重組子，大量雙酶切回收獲得帶粘性末端的山羊fsh - 、亞基基因，將它們分別與同兩個酶大量雙酶切回收獲得的帶粘性末端的pf質粒進行連接，篩選陽性重組子。

One of these recombinants by random chose was fermented. the target protein from the supernatant of bacteria lysate was purified

隨機選取一株rhbfgf表達率約20並有相當可溶性蛋白表達的菌株進行大規模發酵、純化。

The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine. multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol. the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified

轉化嗜甲醇酵母獲得耐受4mg mlg418的菌株，經sds - page電泳和westernblotting檢測表明其上清培養液中的蛋白可與猴抗pzp3抗體和兔抗hcg抗體發生特異性的反應。

Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl

應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。

After identification with both pcr and enzyme digestion methods, accurate recombinants were sent to detect their sequences

將經過pcr和雙酶切鑒定的重組克隆進行序列測定。

Promoter - probe vector psupv4 was used to clone promoters of pseudomonas pseudoalcaligenes in escherichia coli. nine kanamycin resistant recombinants were obtained and designated as ppal - ppa9. the ppa7, which has the highest kanamycin resistance, was chose for further characterization

利用啟動子探針型載體psupv4直接在大腸桿菌中克隆類產堿假單胞菌（ pseudomonaspseudoalcaligenes ）基因啟動子片段，獲得具有強啟動子的重組子ppa7 。

Three sub cloned recombinants were gained and named as ppa7 - 1 ppa7 - 2 ppa7 - 3 each has different inserting fragments. all the three sub cloned recombinants have the same kanamycin resistance about 600u g / ml. the result shows that the pa7 - 2 fragment still has promoter activity. the absence of 0. 9kb from 3 " end had no influence on the promoter activity, while the absence of 0. 7kb from 5 " end led the decrease of kanamycin resistance, which indicates that this 0

卡那黴素抗性測定發現三種次克隆重組子的抗性相似，為600 g ml左右，證明ppa7 ? 2所克隆的5 』端899 - 1120bp片段在大腸桿菌中具有啟動子的功能， 5 』端1120bp后的片段缺失對啟動子沒有影響。 5 』端0 . 7kb片段缺失明顯影響啟動子活性，推測該片段可能對啟動子具有增強的作用。